Urinary excretion profiles of 11-nor-9-carboxy-Delta(9)-tetrahydrocannabinol in humans after single smoked doses of marijuana
Urinary excretion profiles of 11-nor-9-carboxy-Delta(9)-tetrahydrocannabinol in humans after single smoked doses of marijuana
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DOI：
10.1093/jat/20.6.441
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发表时间：
1996-10-01
影响因子：
2.5
通讯作者：
Cone, EJ
中科院分区：
文献类型：
--
作者：
Huestis, MA;Mitchell, JM;Cone, EJ
对大麻尿检呈阳性的解释需要了解人体中大麻代谢物的排泄模式。然而，从大麻使用的对照临床研究中获得的尿排泄数据有限。在这项研究中，六名受试者住在成瘾研究中心的临床病房里，每周抽一支大麻烟（安慰剂，1.75% Δ9-tetrahydrocannabinol [THC]或3.55% THC）。给药后7 d采集个体尿液标本，采用气相色谱-质谱联用（GC-MS）分析11-no -9-羧基-Δ9-tetrahydrocannabinol (THCCOOH)，检出限为0.5 ng/mL。在受试者之间和不同剂量之间，THCCOOH排泄模式在受试者之间存在显著的差异。吸1.75 THC和3.55% THC香烟后，首次尿液中thcooh的平均浓度分别为47±22.3 ng/mL和75.3±48.9 ng/mL。吸烟约15.8 mg和33.8 mg四氢大麻酚后，尿中四氢大麻酚的平均峰值浓度分别为89.8±31.9 ng/mL和153.4±49.2 ng/mL。尿浓度峰值平均时间为1.75%四氢大麻酚组7.7±0.8 h， 3.55%四氢大麻酚组13.9±3.5 h。当截断浓度为15 ng/mL时，吸一枝1.75或3.55% THC香烟后最后一次阳性尿样的平均GC-MS thcooh检测时间分别为33.7±9.2 h和88.6±9.5 h。在吸一支1.75%四氢大麻酚香烟后的7天内，每位受试者平均排出93.9±24.5µg四氢大麻酚（范围34.6-171.6µg）。高剂量后同一时间段内THCCOOH的平均排泄量为197.4±33.6µg（范围107.5 ~ 305.0µg）。这表明，低剂量和高剂量香烟中THC的平均含量分别仅为0.54±0.14%和0.53±0.09%。这些数据提供了服用大麻后尿液中THCCOOH浓度的详细汇编，这可能有助于解释尿大麻素结果。
Interpretation of marijuana-positive urine tests requires an understanding of the excretion pattern of marijuana metabolites in humans. However, limited urinary excretion data from controlled clinical studies of marijuana use are available. In this study, six subjects smoked a single marijuana cigarette (placebo, 1.75% Δ9-tetrahydrocannabinol [THC], or 3.55% THC) each week while residing on the clinical ward of the Addiction Research Center. Individual urine specimens were collected for 7 days after drug administration and analyzed for 11-nor-9-carboxy-Δ9-tetrahydrocannabinol (THCCOOH) by gas chromatography-mass spectrometry (GC-MS) with a limit of detection of 0.5 ng/mL. Substantial intersubject variability in patterns of THCCOOH excretion was noted between subjects and between doses. Mean THCCOOH concentrations in the first urine collections were 47 ± 22.3 ng/mL and 75.3 ± 48.9 ng/mL after the 1.75 and 3.55% THC cigarettes, respectively. Mean peak urine THCCOOH concentrations averaged 89.8 ± 31.9 ng/mL and 153.4 ± 49.2 ng/mL after smoking of approximately 15.8 mg and 33.8 mg THC, respectively. The mean times of peak urine concentration were 7.7 ± 0.8 h after the 1.75% THC and 13.9 ± 3.5 h after the 3.55% THC dose. Mean GC-MS THCCOOH detection times for the last positive urine sample after the smoking of a single 1.75 or 3.55% THC cigarette were 33.7 ± 9.2 h and 88.6 ± 9.5 h, respectively, when a 15-ng/mL cutoff concentration was used. An average of 93.9 ± 24.5 µg THCCOOH (range, 34.6–171.6 µg) was excreted by each subject during the 7-day period after smoking of a single 1.75% THC cigarette. The average amount of THCCOOH excreted in the same time period after the high dose was 197.4 ± 33.6 µg (range, 107.5–305.0 µg). This represented an average of only 0.54 ± 0.14% and 0.53 ± 0.09% of the original amount of THC in the low- and high-dose cigarettes, respectively. These data provide a detailed compilation of THCCOOH concentrations in urine after administration of marijuana that may aid in the interpretation of urine cannabinoid results.