Modular and tunable chemosensor scaffold for divalent zinc
Modular and tunable chemosensor scaffold for divalent zinc
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DOI：
10.1021/ja0355980
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发表时间：
2003-09-03
影响因子：
15
通讯作者：
Imperiali, B
中科院分区：
文献类型：
--
作者：
Shults, MD;Pearce, DA;Imperiali, B
已开发出用于二价锌荧光传感的模块化肽支架。化学传感器的信号成分是螯合敏感荧光团8-羟基-5-(N,N-二甲基磺酰胺基)-2-甲基喹啉，其被制备为受保护的氨基酸衍生物Fmoc-Sox-OH并整合到肽序列中。通过改变 Zn2+ 配体的类型和数量、配体排列以及充当配体之间预组织元件的 β 转角序列，包含信号元件的 19 种合成肽表现出一系列对 Zn2+ 的亲和力。肽-Zn2+复合物的化学计量通过几个标准进行评估。据报道，这些肽对 pH 值和各种重要金属离子的荧光响应。其中 11 个序列仅与 Zn2+ 形成 1:1 复合物，其亲和力范围从 10 nM 到接近 1 muM。当协同使用时，这些传感器可以提供有价值范围内的 Zn2+ 浓度信息。
A modular peptide scaffold has been developed for fluorescent sensing of divalent zinc. The signaling component of the chemosensor is the chelation -sensitive fluorophore 8-hydroxy-5-(N,N-dimethylsulfonamido)-2-methylquinoline, which is prepared as the protected amino acid derivative Fmoc-Sox-OH and integrated into peptide sequences. Nineteen synthetic peptides incorporating the signaling element exhibit a range of affinities for Zn2+ through variation of the type and number of Zn2+ ligands, ligand arrangement and the beta-turn sequence that acts as a preorganization element between the ligands. The stoichiometry of the peptide-Zn2+ complexes is evaluated by several criteria. The fluorescence response of these peptides to pH and various important metal ions is reported. Eleven of these sequences form only 1:1 complexes with Zn2+ and their affinities range from 10 nM to nearly 1 muM. When used in concert, these sensors can provide Zn2+ concentration information in a valuable range.