Effect of growth hormone therapy on nitric oxide formation in cystic fibrosis patients
Effect of growth hormone therapy on nitric oxide formation in cystic fibrosis patients
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DOI：
10.1183/09031936.00103907
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发表时间：
2008-04-01
影响因子：
24.3
通讯作者：
Grasemann, H.
中科院分区：
文献类型：
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作者：
Grasemann, C.;Ratjen, F.;Grasemann, H.
囊性纤维化时气道一氧化氮的产生减少。由于生长激素治疗已被证明可以增加生长激素缺乏患者的一氧化氮产生，因此它也可能影响囊性纤维化患者的一氧化氮产生。本研究的目的是探讨生长激素治疗对囊性纤维化患者全身和气道一氧化氮形成的影响。在囊性纤维化儿童接受人生长激素治疗前、治疗期间和平均1年期间，测量了血清和尿液中的一氧化氮代谢物、血清和痰中的氨基酸浓度以及呼出的一氧化氮。治疗期间，血清和尿液中的一氧化氮代谢物浓度显着增加。治疗期间血清氨基酸浓度（包括 L-精氨酸，一氧化氮合酶的底物）也增加。 L-精氨酸对一氧化氮合酶的全身生物利用度（以L-精氨酸/L-鸟氨酸+赖氨酸的比率表示）保持不变。相比之下，在生长激素治疗期间，痰中的L-精氨酸浓度显着降低，呼出的一氧化氮水平也显着降低。囊性纤维化儿童的生长激素治疗通过降低气道中L-精氨酸的浓度来减少呼出的一氧化氮。
Airway nitric oxide production is decreased in cystic fibrosis. As growth hormone therapy has been shown to increase nitric oxide production in growth hormone-deficient patients, it may also affect nitric oxide production in patients with cystic fibrosis. The objective of the present study was to investigate the effect of growth hormone therapy on systemic and airway nitric oxide formation in patients with cystic fibrosis.Nitric oxide metabolites in serum and urine, amino acid concentrations in serum and sputum as well as exhaled nitric oxide, were measured in children with cystic fibrosis before, during and aver 1 yr of treatment with human growth hormone.Nitric oxide metabolite concentrations increased significantly in serum and urine during the treatment period. Serum amino acid concentrations (including L-arginine, the substrate for nitric oxide synthases) also increased during treatment. The systemic bioavailability Of L-arginine for nitric oxide synthases, expressed as ratio Of L-arginine/L-ornithine+lysine, remained unchanged. In contrast, L-arginine concentrations in sputum decreased significantly during growth hormone treatment, as did exhaled nitric oxide levels.Treatment with growth hormone in children with cystic fibrosis decreases exhaled nitric oxide by reducing the concentration Of L-arginine in the airways.