SPECTROSCOPY AND PHOTOSENSITIZATION OF SAPPHYRINS IN SOLUTIONS AND BIOLOGICAL MEMBRANES
SPECTROSCOPY AND PHOTOSENSITIZATION OF SAPPHYRINS IN SOLUTIONS AND BIOLOGICAL MEMBRANES
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DOI：
10.1111/j.1751-1097.1994.tb05127.x
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发表时间：
1994-11
影响因子：
3.3
通讯作者：
L. Roitman;B. Ehrenberg;Y. Nitzan;V. Král;J. Sessler
中科院分区：
文献类型：
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作者：
L. Roitman;B. Ehrenberg;Y. Nitzan;V. Král;J. Sessler
摘要 提出了三种新的蓝宝石分子的光谱和光物理研究。经过衍生化使其可溶于水的蓝宝石主链具有 700 nm 左右的吸收带，这是生物光敏化所需的特性。我们研究了吸收光谱和荧光光谱，从中获得了在不同极性溶剂中聚集的证据。聚集程度与连接部分的性质相关。以 1,3-二苯基异苯并呋喃为模型目标，测量了单线态氧产生的绝对量子产率，在乙醇中为 0.13-0.18。通过光谱测定与脂质体和细胞的结合常数，并发现其与化合物的疏水性相对应，并具有归因于糖部分的附加效应，这在其中一种蓝宝石的情况下被发现。根据培养混合物中每微克敏化剂杀死的细胞数，蓝宝石蛋白和血卟啉对金黄色葡萄球菌的光损伤效率是相当的。
Abstract A spectroscopic and photophysical study of three new sapphyrin molecules is presented. The sapphyrin backbone that was derivatized to make them water soluble possesses an absorption band around 700 nm, a desired property for biological photosensitization. We studied the absorption and fluorescence spectra, from which evidence for aggregation in solvents of different polarities was obtained. The extent of aggregation is correlated with the nature of the attached moiety. The absolute quantum yields of singlet oxygen production were measured, with 1,3‐diphenyl isobenzofuran as a model target, and were 0.13–0.18 in ethanol. The binding constants to liposomes and to cells were determined spectroscopically and were found to correspond to the hydrophobicities of the compounds, with an additional effect, ascribed to the sugar moiety, which was found in the case of one of the sapphyrins. The efficiency of photodamage to Staphylococcus aureus by sapphyrins and hematoporphyrin was equivalent, on the basis of cells killed per microgram of sensitizer in the incubation mixture.