MOLECULAR CHARACTERIZATION AND BIOLOGICAL FUNCTION OF THE MOVEMENT PROTEIN OF TOBACCO MOSAIC-VIRUS IN TRANSGENIC PLANTS
MOLECULAR CHARACTERIZATION AND BIOLOGICAL FUNCTION OF THE MOVEMENT PROTEIN OF TOBACCO MOSAIC-VIRUS IN TRANSGENIC PLANTS
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DOI：
10.1073/pnas.87.9.3284
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发表时间：
1990-05-01
影响因子：
11.1
通讯作者：
BEACHY, RN
中科院分区：
文献类型：
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作者：
DEOM, CM;SCHUBERT, KR;BEACHY, RN
我们以前证明，在转基因烟草植物中，烟草花叶病毒的运动蛋白（MP）的作用是促进病毒后代在感染过程中的细胞间传播。对这些转基因植株不同组织的分析表明，MP在叶、茎和根组织中积累。在较老的叶子中检测到最高水平。转基因植株叶片组织中MP的相对含量相当于或高于烟草花叶病毒感染叶片组织中MP的含量。转基因叶片组织匀浆的亚细胞分级分离的结果表明，MP是最丰富的老叶的细胞壁部分和蛋白质保持在高水平的细胞壁部分的叶片继续老化。在幼叶中的粗膜/细胞器组分和可溶性组分中检测到显著水平的MP，但在老叶中降低至低水平。这些结果表明MP在细胞壁中积累并稳定。我们以前已经表明，MP修改的分子排斥限制胞间连丝，这是一致的假设，植物病毒从细胞到细胞通过改变胞间连丝。我们在这里表明，烟草花叶病毒MP修改烟草中胞间连丝的分子排斥限制的能力取决于叶片的发育阶段。这些研究结果的影响，了解病毒运动和胞间连丝的功能进行了讨论。
We previously demonstrated, in transgenic tobacco plants, that the role of the movement protein (MP) of tobacco mosaic virus is to facilitate the cell-to-cell spread of viral progeny during infection. An analysis of different tissues of these transgenic plants indicated that the MP accumulated in leaf, stem, and root tissue. The highest levels were detected in older leaves. The relative levels of MP in leaf tissue from transgenic plants were equivalent to, or higher than, the levels of MP in tobacco mosaic virus-infected leaf tissue. Results of subcellular fractionation of homogenates of transgenic leaf tissue showed that the MP was most abundant in the cell wall fraction of older leaves and that the protein remained at high levels in the cell wall fraction as the leaves continued to age. Significant levels of the MP were detected in a crude membrane/organelle fraction and a soluble fraction in younger leaves but decreased to low levels in older leaves. These results suggest that the MP accumulates and is stable in cell walls. We have previously shown that the MP modifies the molecular exclusion limit of plasmodesmata, which is consistent with the hypothesis that plant viruses move from cell to cell through altered plasmodesmata. We show here that the ability of the tobacco mosaic virus MP to modify the molecular exclusion limit of plasmodesmata in tobacco depends on the developmental stage of the leaf. The implications of these findings on understanding virus movement and how plasmodesmata function are discussed.