Engineering glucose swelling responses in poly(N-isopropylacrylamide)-based microgels
Engineering glucose swelling responses in poly(N-isopropylacrylamide)-based microgels
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DOI：
10.1021/ma062254w
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发表时间：
2007-02-06
期刊：
影响因子：
5.5
通讯作者：
Pelton, Robert
中科院分区：
文献类型：
--
作者：
Hoare, Todd;Pelton, Robert
聚（N-异丙基丙烯酰胺）（PNIPAM）微凝胶用氨基苯硼酸（APBA）官能化以产生响应于葡萄糖浓度的增加而溶胀的纳米颗粒。采用“接枝”方法从相同的基础微凝胶合成了一系列具有不同物理性质的微凝胶。随着平台微凝胶中的-COOH基团在表面上变得更加局部化并且在子链内间隔更高，实现了更高的APBA接枝产率。接枝微凝胶的葡萄糖溶胀响应增强，因为PBA官能团变得更加定位于微凝胶的外壳中并且更加随机地分布在凝胶网络子链内。可以利用在PBA-微凝胶缀合物中观察到的葡萄糖诱导的VPTT偏移来产生微凝胶，所述微凝胶表现出作为温度的函数的开关葡萄糖溶胀或在单一可调温度下在特定葡萄糖浓度范围内增强的溶胀响应。因此，应用“次级”热相变以实现“初级”葡萄糖诱导的相变的数量级增强或抑制。随后设计线性和非线性微凝胶葡萄糖传感器，其在目标葡萄糖浓度范围内是活性的。
Poly(N-isopropylacrylamide) (PNIPAM) microgels are functionalized with aminophenyboronic acid (APBA) to produce nanoparticles which swell in response to increases in the glucose concentration. A "graft-to" approach was used to synthesize a range of microgels with different physical properties from the same base microgel. Higher APBA graft yields are achieved as the -COOH groups in the platform microgel become more localized on the surface and more highly spaced within the subchains. The glucose swelling response of the graft microgels is enhanced as the PBA functional groups become more localized in the outer shell of the microgel and more randomly distributed within the gel network subchains. The glucose-induced VPTT shift observed in the PBA-microgel conjugates can be exploited to produce microgels that exhibit on-off glucose swelling as a function of temperature or enhanced swelling responses over specific glucose concentration ranges at a single, tunable temperature. The "secondary" thermal phase transition is thus applied to effect an order-of-magnitude enhancement or suppression of the "primary" glucose-induced phase transition. Both linear and nonlinear microgel glucose sensors are subsequently designed which are active within targeted glucose concentration ranges.