Different core promoters possess distinct regulatory activities in the Drosophila embryo
Different core promoters possess distinct regulatory activities in the Drosophila embryo
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DOI：
10.1101/gad.12.4.547
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发表时间：
1998-02-15
影响因子：
10.5
通讯作者：
Cai, HN
中科院分区：
文献类型：
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作者：
Ohtsuki, S;Levine, M;Cai, HN
有许多共享增强子仅与目标启动子子集相互作用的例子。在一些情况下，特定的增强子-启动子相互作用取决于启动子竞争，由此优选的靶启动子的激活排除了连锁基因的表达。在这里，我们采用转基因胚胎测定来获得启动子选择受 TATA 元件影响的证据。当受到连接的不含 TATA 的启动子攻击时，来自果蝇触角基因复合体 (ANT-C) 的 AE1 增强子和来自 Bithorax 复合体 (BX-C) 的 IAB5 增强子都会优先激活含有 TATA 的启动子。相反，rho 神经外胚层增强子 (NEE) 不区分这两类启动子。因此，某些上游激活剂（例如 Ftz）更喜欢包含 TATA 的启动子，而其他激活剂（包括 Dorsal）对两类启动子都同样有效。这些结果提供了不同核心启动子具有不同调节活性的体内证据。我们讨论了不变的 TFIID 复合物在核心启动子上采用不同构象的可能性。
There are numerous examples of shared enhancers interacting with just a subset of target promoters. In some cases, specific enhancer-promoter interactions depend on promoter competition, whereby the activation of a preferred target promoter precludes expression of linked genes. Here, we employ a transgenic embryo assay to obtain evidence that promoter selection is influenced by the TATA element. Both the AE1 enhancer from the Drosophila Antennapedia gene complex (ANT-C) and the IAB5 enhancer from the Bithorax complex (BX-C) preferentially activate TATA-containing promoters when challenged with linked TATA-less promoters. In contrast, the rho neuroectoderm enhancer (NEE) does not discriminate between these two classes of promoters. Thus, certain upstream activators, such as Ftz, prefer TATA-containing promoters, whereas other activators, including Dorsal, work equally well on both classes of promoters. These results provide in vivo evidence that different core promoters possess distinct regulatory activities. We discuss the possibility that an invariant TFIID complex can adopt different conformations on the core promoter.