Characterization of carotenoid pigments in mature and developing kernels of selected yellow-endosperm sorghum varieties
Characterization of carotenoid pigments in mature and developing kernels of selected yellow-endosperm sorghum varieties
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DOI：
10.1021/jf062939v
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发表时间：
2007-04-04
影响因子：
6.1
通讯作者：
Ferruzzi, Mario G.
中科院分区：
文献类型：
--
作者：
Kean, Ellie G.;Ejeta, Gebisa;Ferruzzi, Mario G.
高粱是撒哈拉以南非洲和印度半干旱地区的重要食物来源，也是膳食植物化学物质（包括类胡萝卜素）的潜在来源。本研究的目的是确定高粱品种的类胡萝卜素配置文件，选择的基础上，他们的黄色胚乳籽粒，在不同的发展阶段。从高粱粉中提取类胡萝卜素后，用高效液相色谱法（HPLC）二极管阵列检测器分离。生理成熟期黄胚乳高粱籽粒总类胡萝卜素含量（0.112- 0.315mg/kg）显著低于黄玉米籽粒（1.152mg/kg）。在完全成熟的高粱品种中观察到总类胡萝卜素和单个类胡萝卜素种类的变化。对于发展中国家的内核，类胡萝卜素含量的大幅增加发生在10和30天后半开花（DAHB），导致在6.06和28.53 μ g的总类胡萝卜素每千粒（TK）之间的峰值积累。从30至50 DAHB观察到显著（p < 0.05）下降，导致最终类胡萝卜素含量为2.62-15.02 μ g/TK总类胡萝卜素。（全-E）-玉米黄质是最丰富的类胡萝卜素，范围从2.22至13.29微克/TK在30 DAHB。（全-E）-β-胡萝卜素的含量适中（0.15-3.83 μ g/TK）。这些数据表明，高粱品种之间存在着发育和成熟籽粒类胡萝卜素积累的遗传变异。
Sorghum is a critical source of food in the semiarid regions of sub-Saharan Africa and India and a potential source of dietary phytochemicals including carotenoids. The objective of this study was to determine the carotenoid profiles of sorghum cultivars, selected on the basis of their yellow-endosperm kernels, at various developmental stages. Following extraction from sorghum flours, carotenoids were separated by high-performance liquid chromatography (HPLC) with diode array detection. Total carotenoid content in fully matured yellow-endosperm sorghum kernels (0.112-0.315 mg/kg) was significantly lower (p < 0.05) than that in yellow maize (1.152 mg/kg) at physiological maturity. Variation in total carotenoids and within individual carotenoid species was observed in fully mature sorghum cultivars. For developing kernels, large increases in carotenoid content occurred between 10 and 30 days after half bloom (DAHB), resulting in a peak accumulation between 6.06 and 28.53 mu g of total carotenoids per thousand kernels (TK). A significant (p < 0.05) decline was noted from 30 to 50 DAHB, resulting in a final carotenoid content of 2.62-15.02 mu g/TK total carotenoids. (all-E)-Zeaxanthin was the most abundant carotenoid, ranging from 2.22 to 13.29 mu g/TK at 30 DAHB. (all-E)-beta-Carotene was present in modest amounts (0.15-3.83 mu g/TK). These data suggest the presence of genetic variation among sorghum cultivars for carotenoid accumulation in developing and mature kernels.