Reduction of analysis time in gas chromatography. Application of low-pressure gas chromatography-tandem mass spectrometry to the determination of pesticide residues in vegetables.
Reduction of analysis time in gas chromatography. Application of low-pressure gas chromatography-tandem mass spectrometry to the determination of pesticide residues in vegetables.
复制标题
减少气相色谱分析时间。
DOI：
10.1016/s0021-9673(03)00887-2
复制
发表时间：
2003
影响因子：
4.1
通讯作者：
N. Sánchez Morito
中科院分区：
文献类型：
--
作者：
F. J. Arrebola;J. L. Martínez Vidal;M. J. González;A. Garrido;N. Sánchez Morito
介绍了一种替代传统毛细管气相色谱法测定蔬菜中农药残留的新方法。本文提出了一种不经净化、二氯甲烷快速提取蔬菜样品的低压气相色谱-串联质谱法。使用上述气相色谱技术，减少了总时间所需的确定72种农药不到一半的时间（31分钟），提高了监测常规实验室的能力。评价了保护柱和将碳玻璃料塞到玻璃衬里中与LP-GC组合的使用。该方法进行了验证，定量限低到足以确定农药残留浓度低于最大残留水平的立法规定。为了评价该方法在真实的样品分析中的适用性，对25个蔬菜样品进行了常规-毛细管GC-MS-MS分析。采用比较技术获得的结果显示，差异低于0.01 mg kg−1。
An alternative to conventional capillary gas chromatography (GC) is evaluated as a new approach to determine pesticide residues in vegetables. Low-pressure gas chromatography–tandem mass spectrometry (LP-GC–MS–MS) is proposed after a fast and simple extraction of the vegetable samples with dichloromethane and without clean up. The use of the above-mentioned GC technique reduced the total time required to determine 72 pesticides to less than half the present time (31 min), increasing the capability of a monitoring routine laboratory. The use of guard column and plug of carbofrit into the glass liner in combination with LP-GC was evaluated. The method was validated with limits of quantitation low enough to determine the pesticide residues at concentrations below the maximum residue levels stated by legislation. In order to assess its applicability to the analysis of real samples, 25 vegetable samples previously determined using conventional-capillary GC–MS–MS were analysed by LP-GC–MS–MS. The results obtained with the compared techniques showed differences lower than 0.01 mg kg−1.