Detection of Helicobacter pylori, Enterococcus faecalis, and Pseudomonas aeruginosa in the subgingival biofilm of HIV-infected subjects undergoing HAART with chronic periodontitis
Detection of Helicobacter pylori, Enterococcus faecalis, and Pseudomonas aeruginosa in the subgingival biofilm of HIV-infected subjects undergoing HAART with chronic periodontitis
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DOI：
10.1007/s10096-009-0786-5
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发表时间：
2009-11-01
影响因子：
4.5
通讯作者：
Colombo, A. P. V.
中科院分区：
文献类型：
--
作者：
Goncalves, L. de Souza;Souto, R.;Colombo, A. P. V.
这项研究比较了患有牙周炎或临床健康牙周组织的 HIV 血清阳性和 HIV 血清阴性受试者的龈下微生物群中幽门螺杆菌、粪肠球菌和铜绿假单胞菌的频率。 54 名受试者被分为两个 HIV 血清阳性组（慢性牙周炎 [HCP = 13] 和牙周健康 [HH = 10]）和两个 HIV 血清阴性组（慢性牙周炎 [CP = 17] 和牙周健康 [H = 14]）。通过聚合酶链反应（PCR）进行细菌种类的检测。 CP患者的牙周破坏、炎症和龈上菌斑明显多于HCP患者（P < 0.05）。所有物种的 CP 和 HCP 患病率均高于 H 个体（P < 0.01）。在 HIV 组中，与健康患者相比，幽门螺杆菌在牙周炎中的患病率显着更高（P < 0.01）。 HH 龈下生物膜中粪肠球菌和铜绿假单胞菌的出现频率高于 H 受试者（P < 0.01）。此外，无论牙周状况如何，与 HIV 血清阴性患者相比，HIV 血清阳性患者中粪肠球菌的检出率显着更高（P < 0.01）。这些数据表明，在牙周炎受试者的龈下微生物群中经常检测到幽门螺杆菌。相比之下，患有牙周炎或牙周健康的艾滋病毒血清阳性患者的粪肠球菌患病率较高。
This study compared the frequency of Helicobacter pylori, Enterococcus faecalis, and Pseudomonas aeruginosa in the subgingival microbiota of HIV-seropositive and HIV-seronegative subjects with periodontitis or clinically healthy periodontal tissues. Fifty-four subjects were distributed into two HIV-seropositive groups (chronic periodontitis [HCP = 13] and periodontal health [HH = 10]) and two HIV-seronegative groups (chronic periodontitis [CP = 17] and periodontal health [H = 14]). The detection of bacterial species was carried out by polymerase chain reaction (PCR). CP patients showed significantly more periodontal destruction, inflammation, and supragingival plaque than HCP patients (P < 0.05). All species were detected at a higher prevalence in CP and HCP than H individuals (P < 0.01). In the HIV groups, H. pylori was significantly more prevalent in periodontitis compared to healthy patients (P < 0.01). A higher frequency of E. faecalis and P. aeruginosa was observed in the subgingival biofilm of HH than H subjects (P < 0.01). Moreover, E. faecalis was detected significantly more often in HIV-seropositive compared to HIV-seronegative patients, regardless of periodontal status (P < 0.01). These data indicate that H. pylori is frequently detected in the subgingival microbiota of periodontitis subjects. In contrast, HIV-seropositive patients with either periodontitis or periodontal health present a high prevalence of E. faecalis.