Serum EBV antibodies and LMP-1 in Polish patients with oropharyngeal and laryngeal cancer.
Serum EBV antibodies and LMP-1 in Polish patients with oropharyngeal and laryngeal cancer.
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DOI：
10.1186/s13027-017-0141-x
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发表时间：
2017
影响因子：
3.7
通讯作者：
Polz-Dacewicz M
中科院分区：
文献类型：
--
作者：
Fołtyn S;Strycharz-Dudziak M;Drop B;Boguszewska A;Polz-Dacewicz M
许多研究者报道了eb病毒（EBV）与头颈癌发展之间的关系。本研究的目的是检测110名波兰口咽喉癌患者的EBV DNA和EBV抗体，并与40名健康个体进行比较。冷冻肿瘤组织片段采用巢式PCR法检测EBV DNA。采用ELISA检测所有个体血清中VCA IgM和IgG、EBNA IgG、EA IgG的存在。52.7%的患者检测到EBV DNA（对照组为25%）。EBVCA阳性率为94.5%，EBNA阳性率为96.4%，EA阳性率为94.5%。患者EA水平明显升高提示EBV再激活。大多数患者（83%）感染了野生型EBV。我们的研究表明，这种变异似乎与波兰人口的口咽癌和喉癌有关。
The association between Epstein-Barr virus (EBV) and the development of head and neck cancer was reported by many researchers. The aim of the present study was to detect EBV DNA and EBV antibodies in 110 Polish patients with oropharyngeal and laryngeal cancer compared to 40 healthy individuals. Frozen tumor tissue fragments were tested using nested PCR assay for EBV DNA detection. Sera from all individuals were investigated using ELISA tests to detect the presence of VCA IgM and IgG, EBNA IgG, EA IgG. EBV DNA was detected in 52.7% of the patients (25% in controls). EBVCA were detected in 94.5%, EBNA in 96.4% and EA in 94.5% of patients. The significantly higher level of EA in the patients suggests EBV reactivation. The majority of patients (83%) were infected with wild-type EBV. Our study showed that this variant seems to be associated with oropharyngeal and laryngeal cancer in the Polish population.