Molecular characterization of Borrelia spp. isolates from greater metropolitan Chicago reveals the presence of Borrelia bissettii. Preliminary report.
Molecular characterization of Borrelia spp. isolates from greater metropolitan Chicago reveals the presence of Borrelia bissettii. Preliminary report.
复制标题
疏螺旋体属的分子表征。
DOI：
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发表时间：
2000
影响因子：
1.2
通讯作者：
M. Picken
中科院分区：
文献类型：
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作者：
R. Picken;M. Picken
莱姆病在美国集中在三个流行地区：东北部、中西部上部和太平洋海岸。在中西部，莱姆病的范围已经扩大到威斯康星州和明尼苏达州的大部分地区。尽管伊利诺伊州靠近中西部聚集区，但到目前为止，它还没有被认为是地方病流行地区。然而，最近的数据表明，这种情况可能正在改变。此外，中西部地区的硼砂多样性在很大程度上仍未得到开发。在这里，我们介绍了从库克县和莱克县捕获的啮齿动物分离的疏螺旋体的分子特征的初步结果，这两个县都是伊利诺伊州芝加哥大都市地区的一部分。我们调查了芝加哥郊区森林地区存在的啮齿动物宿主，以便通过培养分离螺旋体来确定莱姆病病剂(S)感染的频率(Picken等人，未发表)。通过遗传特征对疏螺旋体啮齿动物分离株进行了种水平鉴定。总共从NW Cook公司和Lake公司获得了19个分离株，脉冲场凝胶电泳法分析这些分离物的Mlu1酶切图谱显示，这些分离物与加州分离株DN127(PF类型I)、纽约分离株25015(PF类型II)或后者的变异体(PF类型III)相似。从核糖体RNA基因簇的RRF(5S)-RRL(23S)基因间隔区产生的序列数据证实了到目前为止研究的所有芝加哥分离株的身份为B.Bissettii。这些菌株不同于我们之前从伊利诺伊州西北部和威斯康星州分离的疏螺旋体。此外，白纹伊蚊的感染与孕期啮齿动物种类如田鼠和乌龟有明显的相关性。这一新的地方性疫源地与已建立的莱姆病中西部地方性疫源地的关系仍有待阐明。到目前为止，这种生物的地理范围和水库多样性可能被低估了。
Lyme Disease in the US is concentrated in three endemic areas: the Northeast, the upper mid-West, and the Pacific coast. In the mid-West, the range of Lyme disease has expanded to include large parts of Wisconsin and Minnesota. Despite its proximity to the mid-Western focus, Illinois, so far, has not been considered an endemic area. However, more recent data suggest that this situation may be changing. Also, the extent of borrelial diversity in the mid-West remains largely unexplored. Here, we present preliminary results on the molecular characterization of Borrelia isolates from rodents captured in Cook and Lake Counties, both of which are parts of the greater metropolitan Chicago area in Illinois. We investigated the rodent reservoir present in forested areas of suburban Chicago in order to determine the frequency of infection with the Lyme disease agent(s) by culture isolation of Borrelia spirochetes (Picken et al., unpublished). Rodent isolates of Borrelia were identified to the species level by genetic characterization. In total, 19 isolates were obtained over 3 years from NW Cook Co. and Lake Co. Pulsed-field gel electrophoretic analysis of Mlul digested DNA from these isolates showed macrorestriction patterns similar to that of the Californian isolate, strain DN127 (PF type I), New York isolate strain 25015 (PF type II), or a variant of the latter (PF type III). Sequence data generated from the rrf(5S)-rrl(23S) intergenic spacer region of the ribosomal RNA gene cluster confirmed the identity of all the Chicago isolates studied to date as B. bissettii. These strains are unlike our previous Borrelia isolates from NW Illinois and Wisconsin. In addition, there was a predominant association of B. bissettii infection with pratal rodent species such as Microtus pennsylvanicus and Zapus hudsonius. The relationship of this novel enzootic focus to the established mid-Western endemic focus of Lyme disease remains to be elucidated. The geographic range and reservoir diversity of this organism may have hitherto been underestimated.