Neuropeptide-Y Y2-receptor agonist, PYY3-36 promotes non-rapid eye movement sleep in rat
Neuropeptide-Y Y2-receptor agonist, PYY3-36 promotes non-rapid eye movement sleep in rat
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DOI：
10.1016/j.neures.2005.11.006
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发表时间：
2006-03-01
影响因子：
2.9
通讯作者：
Honda, K
中科院分区：
文献类型：
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作者：
Akanmu, MA;Ukponmwan, OE;Honda, K
PYY3-36是肠道-脑轴的主要成分，外周给药对摄食、脑功能有显著影响，对神经肽Y-2受体有更强的选择性。因此，我们研究了夜间单次腹腔注射PYY3-36(30和100微克/公斤体重)的效果。对大鼠的食物摄入量、水摄入量和睡眠-觉醒周期的影响。对植入皮层脑电(EEG)和颈部肌电(EMG)电极的雄性SD大鼠进行睡眠记录。测定脑电、肌电、食物摄入量和水摄入量。脑电记录分为快速眼动(REM)睡眠、非快速眼动(NREM)睡眠和觉醒。PYY3-36在天黑开始前15分钟给予PYY3-36显著增加非快速眼动(NREM)睡眠和减少觉醒。对每隔4小时的暗期的分析表明，夜间给予PYY3-36(30和100微克/公斤)可显著抑制觉醒，增加前4小时的非快速眼动睡眠。与给药前相比，PYY3-36(30和100微克/公斤)给药后，小鼠觉醒时间明显缩短。此外，PYY3-36(30和100µg/kg ip)导致在NREM睡眠中花费的时间增加。小剂量PYY3-36(30µg/kg)夜间给药也可显著减少小鼠的摄食量[F(2，23)=4.90，p<；0.05]，但对摄食量无明显影响。这些结果提示，PYY3-36可能在促进NREM睡眠和进食行为中起重要作用。(C)2005年爱思唯尔爱尔兰有限公司和日本神经科学学会。版权所有。
PYY3-36 is a major component of the gut-brain axis and peripheral administration has been reported to exert significant effects on feeding, brain function and is more selective for neuropeptide Y-2 receptor. Therefore, we investigated the effects of nocturnal intraperitoneal administration of single doses of PYY3-36 (30 and 100 mu g/kg i.p.) on food intake, water intake and the sleep-wake cycle in rats. Sleep recordings were carried out in male Sprague-Dawley rats implanted with cortical electroencephalogram (EEG) and neck electromyograrn (EMG) electrodes. The EEG, EMG, food intake and water intake were assessed. The electrographic recordings obtained were scored visually as rapid eye movement (REM) sleep, non-REM (NREM) sleep and wakefulness.PYY3-36 administration 15 min prior to dark onset significantly (p < 0.05) increased non-rapid eye movement (NREM) sleep and decreased wakefulness. Analysis of the dark-period at 4-h time intervals showed that nocturnal administration of PYY3-36 (30 and 100 mu g/kg) significantly suppressed wakefulness and increased non-REM sleep during the first 4-h time interval. Time spent in wakefulness was significantly decreased after administration of PYY3-36 (30 and 100 mu g/kg) when compared with administration of vehicle. In addition, PYY3-36 (30 and 100 mu g/kg i.p.) induced an increase in the time spent in NREM sleep. The nocturnal intraperitoneal administration of the lower dose of PYY3-36 (30 mu g/kg) also significantly decreased food intake [F (2,23) = 4.90, p < 0.05] but had no effect on water intake. These findings suggest that PYY3-36 may play an important role in the enhancement of NREM sleep and feeding behavior. (c) 2005 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.