Ligands playing musical chairs with G-quadruplex DNA: A rapid and simple displacement assay for identifying selective G-quadruplex binders
Ligands playing musical chairs with G-quadruplex DNA: A rapid and simple displacement assay for identifying selective G-quadruplex binders
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DOI：
10.1016/j.biochi.2008.02.019
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发表时间：
2008-08-01
期刊：
影响因子：
3.9
通讯作者：
Teulade-Fichou, M. -R
中科院分区：
文献类型：
--
作者：
Monchaud, D.;Allain, C.;Teulade-Fichou, M. -R
我们在这里报告的G4-FLD（G-四链体荧光嵌入剂置换），一个简单的方法，旨在评估四链体DNA结合亲和力和四链体的推定配体的DNA选择性的细节。该测定法基于噻唑橙子从四链体和双链体DNA基质中置换后的荧光损失。最初的协议进行了测试，使用不同的四链体和DNA的目标，并与广泛的面板G-四链体配体属于不同的家庭（即从喹吖啶类金属有机配体）可能会显示各种结合模式。通过与FRET-熔解和ESI-MS测定法的比较，进一步支持了测定法的可靠性。(C)2008年，Elsevier Masson SAS。All rights reserved.
We report here the details of G4-FlD (G-quadruplex fluorescent intercalator displacement), a simple method aiming at evaluating quadruplex-DNA binding affinity and quadruplex-over duplex-DNA selectivity of putative ligands. This assay is based on the loss of fluorescence upon displacement of thiazole orange from quadruplex- and duplex-DNA matrices. The original protocol was tested using various quadruplex- and duplex-DNA targets, and with a wide panel of G-quadruplex ligands belonging to different families (i.e. from quinacridines to metallo-organic ligands) likely to display various binding modes. The reliability of the assay is further supported by comparisons with FRET-melting and ESI-MS assays. (C) 2008 Elsevier Masson SAS. All rights reserved.