Polysaccharide degradation by lytic polysaccharide monooxygenases
Polysaccharide degradation by lytic polysaccharide monooxygenases
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DOI：
10.1016/j.sbi.2019.02.015
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发表时间：
2019-12-01
影响因子：
6.8
通讯作者：
Eijsink, Vincent G. H.
中科院分区：
文献类型：
--
作者：
Forsberg, Zarah;Sorlie, Morten;Eijsink, Vincent G. H.
通过目前已知为溶解性多糖单加氧酶（LPMO）的酶氧化裂解糖苷键的发现对我们目前对柠檬酸多糖如几丁质和纤维素的酶促转化的理解具有重大影响。LPMO序列家族的数量不断扩大，新的底物特异性和生物学功能正在被发现。这些LPMO的催化机制仍然有些神秘。最近，新的见解已经从研究中获得的酶-底物复合物的X-射线晶体学，EPR，NMR和建模。此外，它已被证明，LPMO可以进行过氧合酶反应，在比单加氧酶反应，这影响了我们的理解和利用这些强大的酶高得多的速度。
The discovery of oxidative cleavage of glycosidic bonds by enzymes currently known as lytic polysaccharide monooxygenases (LPMOs) has had a major impact on our current understanding of the enzymatic conversion of recalcitrant polysaccharides such as chitin and cellulose. The number of LPMO sequence families keeps expanding and novel substrate specificities and biological functionalities are being discovered. The catalytic mechanism of these LPMOs remains somewhat enigmatic. Recently, novel insights have been obtained from studies of enzyme-substrate complexes by X-ray crystallography, EPR, NMR, and modeling. Furthermore, it has been shown that LPMOs may carry out peroxygenase reactions, at much higher rates than monooxygenase reactions, which affects our understanding and exploitation of these powerful enzymes.