Prenatal LHRH neurons in nasal explant cultures express estrogen receptor beta transcript.
Prenatal LHRH neurons in nasal explant cultures express estrogen receptor beta transcript.
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DOI：
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发表时间：
2002
期刊：
影响因子：
4.8
通讯作者：
N. Sharifi;Andreé E. Reuss;S. Wray
中科院分区：
文献类型：
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作者：
N. Sharifi;Andreé E. Reuss;S. Wray
性类固醇影响LHRH神经元活动，根据动物的生殖状态，发挥负反馈或正反馈作用。最近的证据表明，LHRH神经元具有雌激素受体β（ER β）亚型出生后，表明雌激素可能会直接作用于LHRH神经元的一部分。在这项研究中，我们确定ER β转录在产前LHRH神经元作为年龄的函数。单细胞cDNA池由LHRH神经元在体外维持7、14和28天（div）制成。用ER β特异性引物通过PCR筛选cDNA库，发现所有三个年龄段的ER β阳性LHRH神经元。但在14（6/10）~ 28 div（1/10）时，共表达ER β转录本的LHRH细胞数量明显减少。没有一个LHRH细胞的ER α转录阳性。这些结果表明，发育中的LHRH神经元表达ER β的转录本，并表明ER β的持续表达是LHRH神经元的特征，其可能需要来自中枢神经系统和/或外周的线索，或者是预定要在LHRH神经元的亚群中维持的。
Sex steroids influence LHRH neuronal activity, exerting a negative or positive feedback action, depending on the reproductive state of the animal. Recent evidence indicates that LHRH neurons possess the estrogen receptor beta (ERbeta) subtype postnatally, suggesting that estrogen may act, in part, directly on LHRH neurons. In this study, we identified ERbeta transcript in prenatal LHRH neurons as a function of age. Single-cell cDNA pools were made from LHRH neurons maintained for 7, 14, and 28 d in vitro (div). Screening of the cDNA pools by PCR with ERbeta-specific primers revealed ERbeta-positive LHRH neurons at all three ages. However, the number of LHRH cells coexpressing ERbeta transcript decreased dramatically between 14 (6/10) and 28 div (1/10). None of the LHRH cells were positive for ERalpha transcript. These results indicate that developing LHRH neurons express the transcript for ERbeta and suggest that continued expression of ERbeta is either a characteristic of LHRH neurons that may require cues from the central nervous system and/or periphery or predetermined to be maintained in a subpopulation of LHRH neurons.