The RNA product of the Doc retrotransposon is localized on the Drosophila oocyte cytoskeleton
The RNA product of the Doc retrotransposon is localized on the Drosophila oocyte cytoskeleton
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DOI：
10.1007/s004380050675
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发表时间：
1998-03
期刊：
影响因子：
--
通讯作者：
D. Zhao;M. Bownes
中科院分区：
文献类型：
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作者：
D. Zhao;M. Bownes
果蝇核糖体的单反转录转座子编码一个带有两个开放阅读框架(ORF)的RNA。ORF1编码一个RNA结合蛋白，并与编码逆转录酶的ORF2重叠。反转录转座子通过全长RNA中间体在基因组中转座到新的位置。因为它们不编码外壳蛋白，转座仅限于单细胞谱系。在这篇文章中，我们提出的证据表明，Doc转录本水平最高的是成年男性和女性生殖系。DocRNA定位于果蝇细胞的细胞骨架区域。这一现象的生物学意义尚不清楚，但我们将讨论其中的一些可能性。
TheDocretrotransposon ofDrosophilamelanogasterencodes an RNA with two open reading frames (ORFs). ORF1 encodes an RNA-binding protein and overlaps with ORF2, which encodes a reverse transcriptase. Retrotransposons transpose to new positions in the genome via a full-length RNA intermediate. Because they do not encode a coat protein, transposition is limited to single cell lineages. In this paper we present evidence that shows that the highest levels of Doc transcripts are found in the adult male and female germ-line. TheDocRNA is localized in the region of the cytoskeleton of theDrosophilaoocyte. The biological significance of this is unknown, but we discuss some of the possibilities.