The plasmids found in isolates of the acidothermophilic archaebacterium Thermoplasma acidophilum

The plasmids found in isolates of the acidothermophilic archaebacterium Thermoplasma acidophilum
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在嗜酸嗜热古细菌嗜酸热原体分离株中发现的质粒

DOI:
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发表时间:
1995
期刊:
影响因子:
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通讯作者:
T. Oshima
T. Oshima
中科院分区:
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文献类型:
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作者:
M. Yasuda;A. Yamagishi;T. Oshima

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在嗜酸嗜热古细菌嗜酸热浆菌的分离株中检测到质粒酶。对其中一种质粒pTA 1进行了表征。质粒为15.2kbp的环状DNA。使用三种限制性内切酶构建物理图谱。该质粒的拷贝数估计为每个细胞7-13个。在宿主细胞的染色体DNA中未发现同源序列。
Plasmids were detected in isolates of an acidothermophilic archaebacterium Thermoplasma acidophilum. One of the plasmids, pTA1, was characterized. The plasmid was a circular DNA of 15.2 kbp. A physical map was constructed using three restriction endonucleases. A copy number of this plasmid was estimated to be 7–13 per cell. The homologous sequence was not found in the chromosomal DNA of the host cell.