Protochlorophyllide: a new photosensitizer for the photodynamic inactivation of Gram-positive and Gram-negative bacteria.
Protochlorophyllide: a new photosensitizer for the photodynamic inactivation of Gram-positive and Gram-negative bacteria.
复制标题
原叶绿素：一种新型光敏剂，用于光动力灭活革兰氏阳性和革兰氏阴性细菌。
作者：
J. Walther;M. Bröcker;Denise Wätzlich;M. Nimtz;M. Rohde;D. Jahn;J. Moser
对抗生素的耐药性不断增加，需要寻找替代治疗策略。光动力疗法是一种很有前途的治疗方法。叶绿素生物合成的天然中间体，原叶绿素，生产，纯化和测试作为一种新的光敏剂的灭活五个模式生物，包括金黄色葡萄球菌，单核细胞增生李斯特菌和假结核耶尔森氏菌，所有负责严重的临床感染。当微生物暴露于钨丝灯的白色光（0.1 mW cm（-2））下时，革兰氏阳性S.金黄色L.单核细胞增多症和枯草芽孢杆菌在0.5 mg L（-1）原叶绿素浓度下被光化学灭活。透射电子显微镜显示，在细胞分裂和细胞质细胞内容物的部分损失的隔膜形成紊乱。革兰氏阴性Y.假结核和大肠杆菌被发现是不敏感的原叶绿素治疗由于外膜的渗透性屏障。然而，分别加入50和20 mg L（-1）的多粘菌素B九肽后，这两种细菌对原叶绿素（10 mg L（-1））的根除敏感。观察到DNA的释放和细胞质的有害重排。
The growing resistance against antibiotics demands the search for alternative treatment strategies. Photodynamic therapy is a promising candidate. The natural intermediate of chlorophyll biosynthesis, protochlorophyllide, was produced, purified and tested as a novel photosensitizer for the inactivation of five model organisms including Staphylococcus aureus, Listeria monocytogenes and Yersinia pseudotuberculosis, all responsible for serious clinical infections. When microorganisms were exposed to white light from a tungsten filament lamp (0.1 mW cm(-2)), Gram-positive S. aureus, L. monocytogenes and Bacillus subtilis were photochemically inactivated at concentrations of 0.5 mg L(-1) protochlorophyllide. Transmission electron microscopy revealed a disordered septum formation during cell division and the partial loss of the cytoplasmic cell contents. Gram-negative Y. pseudotuberculosis and Escherichia coli were found to be insensitive to protochlorophyllide treatment due to the permeability barrier of the outer membrane. However, the two bacteria were rendered susceptible to eradication by protochlorophyllide (10 mg L(-1)) upon addition of polymyxin B nonapeptide at 50 and 20 mg L(-1), respectively. The release of DNA and a detrimental rearrangement of the cytoplasm were observed.
影响因子：
4.8
作者：
Broecker, Markus J.;Virus, Simone;Moser, Juergen
通讯作者：
Moser, Juergen