ENZYMATIC ASSAYS FOR 2-DEOXYGLUCOSE AND 2-DEOXYGLUCOSE 6-PHOSPHATE
ENZYMATIC ASSAYS FOR 2-DEOXYGLUCOSE AND 2-DEOXYGLUCOSE 6-PHOSPHATE
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DOI：
10.1016/0003-2697(87)90481-7
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发表时间：
1987-03-01
影响因子：
2.9
通讯作者：
LOWRY, OH
中科院分区：
文献类型：
--
作者：
CHI, MMY;PUSATERI, ME;LOWRY, OH
描述了用于2-脱氧葡萄糖（2-DG）和2-脱氧葡萄糖6-磷酸（DG 6P）的方法，其基于以下事实：DG 6P被葡萄糖-6-磷酸脱氢酶（G6 PDH）氧化，但速率比葡萄糖6-磷酸慢1000倍，而己糖激酶以相当的速率磷酸化2DG和葡萄糖。因此，通过以适当的顺序和适当的浓度添加两种酶，2DG、葡萄糖、DG 6P和葡萄糖6-P都可以分别测量。为了避免使用高水平G6 PDH的副反应，当测量DG 6P时，首先用葡萄糖氧化酶加醛糖还原酶除去葡萄糖。
Methods for 2-deoxyglucose (2-DG) and 2-deoxyglucose 6-phosphate (DG6P) are described which are based on the fact that DG6P is oxidized by glucose-6-phosphate dehydrogenase (G6PDH), but at a rate 1000-fold slower than for glucose 6-phosphate, whereas hexokinase phosphorylates 2DG and glucose at comparable rates. Therefore, by adding the two enzymes in a suitable order, and in appropriate concentrations, 2DG, glucose, DG6P, and glucose 6-P can all be separately measured. To avoid a side reaction from the use of a high level of G6PDH, when measuring DG6P, glucose is first removed with glucose oxidase plus aldose reductase.