Induction of protective immunity against severe acute respiratory syndrome coronavirus (SARS-CoV) infection using highly attenuated recombinant vaccinia virus DIs
Induction of protective immunity against severe acute respiratory syndrome coronavirus (SARS-CoV) infection using highly attenuated recombinant vaccinia virus DIs
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DOI：
10.1016/j.virol.2006.03.020
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发表时间：
2006-08-01
期刊：
影响因子：
3.7
通讯作者：
Tsunetsugu-Yokota, Yasuko
中科院分区：
文献类型：
--
作者：
Ishii, Koji;Hasegawa, Hideki;Tsunetsugu-Yokota, Yasuko
SARS冠状病毒（SARS-CoV）是近年来发现的SARS的病原体。我们构建了一系列重组的DIs（rDIs），一种高度减毒的牛痘病毒株，其单独或同时表达编码SARS-CoV的四种结构蛋白（E、M、N和S）的基因。这些rDI在鼻内或皮下给药后在接种疫苗的小鼠中引起SARS-CoV特异性血清IgG抗体和T细胞应答。皮下接种表达S蛋白（含或不含其他结构蛋白）的rDI的小鼠诱导了高水平的血清中和IgG抗体，并在不存在粘膜伊加应答的情况下表现出对SARS-CoV攻击的显著保护性免疫。这些结果表明，皮下注射含S的rDI引起的有效免疫应答能够控制SARS-CoV的粘膜感染。因此，复制缺陷型DI构建体有望开发安全有效的SARS疫苗。(c)2006年爱思唯尔公司All rights reserved.
SARS-coronavirus (SARS-CoV) has recently been identified as the causative agent of SARS. We constructed a series of recombinant DIs (rDIs), a highly attenuated vaccinia strain, expressing a gene encoding four structural proteins (E, M, N and S) of SARS-CoV individually or simultaneously. These rDIs elicited SARS-CoV-specific serum IgG antibody and T-cell responses in vaccinated mice following intranasal or subcutaneous administration. Mice that were subcutaneously vaccinated with rDIs expressing S protein with or without other structural proteins induced a high level of serum neutralizing IgG antibodies and demonstrated marked protective immunity against SARS-CoV challenge in the absence of a mucosal IgA response. These results indicate that the potent immune response elicited by subcutaneous injection of rDIs containing S is able to control mucosal infection by SARS-CoV. Thus, replication-deficient DIs constructs hold promise for the development of a safe and potent SARS vaccine. (c) 2006 Elsevier Inc. All rights reserved.