Saliva TwoStep for rapid detection of asymptomatic SARS-CoV-2 carriers.
Saliva TwoStep for rapid detection of asymptomatic SARS-CoV-2 carriers.
复制标题
DOI：
10.7554/elife.65113
复制
发表时间：
2021-03-29
期刊：
影响因子：
7.7
通讯作者：
Sawyer SL
中科院分区：
文献类型：
--
作者：
Yang Q;Meyerson NR;Clark SK;Paige CL;Fattor WT;Gilchrist AR;Barbachano-Guerrero A;Healy BG;Worden-Sapper ER;Wu SS;Muhlrad D;Decker CJ;Saldi TK;Lasda E;Gonzales P;Fink MR;Tat KL;Hager CR;Davis JC;Ozeroff CD;Brisson GR;McQueen MB;Leinwand LA;Parker R;Sawyer SL
在这里，我们开发了一个简单的分子测试SARS-CoV-2唾液的基础上逆转录环介导的等温扩增。该测试有两个步骤：（1）用稳定溶液加热唾液和（2）通过与引物/酶混合物孵育来检测病毒。培养后，含有SARS-CoV-2基因组的唾液样本变成亮黄色。因为这个测试是pH值依赖性的，它可能会对一些天然酸性唾液样本产生错误的反应。我们报告了独特的唾液稳定协议，使295个健康唾液样本与测试兼容，产生零假阳性。我们还评估了278份唾液样本的测试，这些样本来自感染SARS-CoV-2但在唾液收集时没有症状的个体，以及来自感染个体的54对配对唾液和前鼻样本。本文所述的唾液两步测试在具有亚临床（无症状或症状前）感染的个体中以94%的灵敏度和>99%的特异性鉴定感染。
Here, we develop a simple molecular test for SARS-CoV-2 in saliva based on reverse transcription loop-mediated isothermal amplification. The test has two steps: (1) heat saliva with a stabilization solution and (2) detect virus by incubating with a primer/enzyme mix. After incubation, saliva samples containing the SARS-CoV-2 genome turn bright yellow. Because this test is pH dependent, it can react falsely to some naturally acidic saliva samples. We report unique saliva stabilization protocols that rendered 295 healthy saliva samples compatible with the test, producing zero false positives. We also evaluated the test on 278 saliva samples from individuals who were infected with SARS-CoV-2 but had no symptoms at the time of saliva collection, and from 54 matched pairs of saliva and anterior nasal samples from infected individuals. The Saliva TwoStep test described herein identified infections with 94% sensitivity and >99% specificity in individuals with sub-clinical (asymptomatic or pre-symptomatic) infections.