Antiviral function of tilapia hepcidin 1-5 and its modulation of immune-related gene expressions against infectious pancreatic necrosis virus (IPNV) in Chinook salmon embryo (CHSE)-214 cells.
Antiviral function of tilapia hepcidin 1-5 and its modulation of immune-related gene expressions against infectious pancreatic necrosis virus (IPNV) in Chinook salmon embryo (CHSE)-214 cells.
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DOI：
10.1016/j.fsi.2010.09.005
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发表时间：
2011
影响因子：
4.7
通讯作者：
V. Rajanbabu;Jyh-Yih Chen
中科院分区：
文献类型：
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作者：
V. Rajanbabu;Jyh-Yih Chen
抗菌肽是一类富含半胱氨酸的小分子物质，在宿主抵御病原体感染的防御机制中发挥着重要作用。近年来，罗非鱼海普西丁(TH)1-5进行了鉴定，并报道了其对多种病原菌的抑菌作用。为此，我们在Chinook鲑鱼胚胎(CHSE)-214细胞上研究了TH1-5对传染性胰腺坏死病毒(IPNV)的抗病毒作用。TH1-5的存在提高了感染IPNV的CHSE-214细胞的存活。此外，当IPNV与TH1-5预先孵育时，IPNV在CHSE-214细胞中由细胞病变效应形成的斑块数量减少。这一观察结果证明了TH1-5的抗病毒功能。实时聚合酶链式反应研究表明，TH1-5对白细胞介素性、膜联蛋白和其他病毒应答基因的表达有调节作用。用TH1-5和IPNV共处理CHSE-214细胞，再用IPNV攻击细胞24小时后，细胞不能存活。这表明在没有TH1-5的情况下，病毒的再次攻击杀死了CHSE-214细胞。结论：TH1-5通过直接抑菌和免疫调节作用保护CHSE-214细胞免受IPNV感染。
Antimicrobial peptides, small cysteine-rich molecules, play vital roles in host defense mechanisms against pathogen infection. Recently, tilapia hepcidin (TH)1–5, was characterized, and its antimicrobial functions against several pathogens were reported. Herein, we investigated the antiviral functions of TH1-5 against infectious pancreatic necrosis virus (IPNV) in Chinook salmon embryo (CHSE)-214 cells. The presence of TH1-5 enhanced the survival of CHSE-214 cells infected with IPNV. Additionally, the number of plaques formed by the cytopathic effect of IPNV in CHSE-214 cells decreased when IPNV was preincubated with TH1-5. This observation demonstrates the antiviral function of TH1-5. Real-time PCR studies showed the modulation of interleukin, annexin, and other viral-responsive gene expressions by TH1-5. When TH1-5 and IPNV were used to co-treat CHSE-214 cells, then cells were re-challenged with IPNV at 24h, the cells did not survive the IPNV infection. This shows that in the absence of TH1-5, viral re-challenge killed CHSE-214 cells. In conclusion TH1-5 protected CHSE-214 cells against IPNV by direct antimicrobial and immunomodulatory functions.