Genetic diversity of Enterococcus faecalis isolated from environmental, animal and clinical sources in Malaysia
Genetic diversity of Enterococcus faecalis isolated from environmental, animal and clinical sources in Malaysia
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DOI：
10.1016/j.jiph.2017.02.006
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发表时间：
2017-09-01
影响因子：
6.7
通讯作者：
Rahman, Sadequr
中科院分区：
文献类型：
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作者：
Daniel, Diane S.;Lee, Sui M.;Rahman, Sadequr
粪肠球菌是引起医院感染的主要原因之一。据报道，栖息于动物体内的粪肠球菌与环境来源之间存在强烈的流行病学联系。本研究调查了马来西亚三个来源的粪肠球菌的遗传多样性、抗生素耐药性和毒力决定因素。共分离粪肠球菌250株，其中家畜分离株120株，水源分离株100株，住院病人分离株30株。脉冲场凝胶电泳法检测到63种脉冲型，所有来源均表现出高度的多样性(D=0.901)。这三个来源没有共同的脉冲型。每个病房都有自己独特的PFGE模式，这种模式在六个月后仍然存在。测定了万古霉素、庆大霉素、青霉素、四环素、呋喃妥因、左氧氟沙星、环丙沙星和磷霉素的最低抑菌浓度。对四环素的耐药性最普遍的是来自农场动物(62%)和水源(49%)的分离株。水分离株(86%)有更高的asal基因，编码聚集物质，而临床分离株(78%)和农场动物分离株(87%)有更高的esp基因，编码表面暴露蛋白。这项研究产生了马来西亚不同来源的具有抗生素耐药性和毒力特征的粪肠球菌的遗传多样性的知识。(C)2017年作者。爱思唯尔有限公司出版。
Enterococcus faecalis ranks as one of the leading causes of nosocomial infections. A strong epidemiological link has been reported between E. faecalis inhabiting animals and environmental sources. This study investigates the genetic diversity, antibiotic resistance and virulence determinants in E. faecalis from three sources in Malaysia. A total of 250 E. faecalis isolates were obtained consisting of 120 isolates from farm animals, 100 isolates from water sources and 30 isolates from hospitalized patients. Pulse-field gel electrophoresis-typing yielded 63 pulsotypes, with high diversity observed in all sources (D =>= 0.901). No pulsotype was common to all the three sources. Each patient room had its own unique PFGE pattern which persisted after six months. Minimum inhibitory concentrations of Vancomycin, Gentamicin, Penicillin, Tetracycline, Nitrofurantoin, Levofloxacin, Ciprofloxacin and Fosfomycin were evaluated. Resistance to Tetracycline was most prevalent in isolates from farm animals (62%) and water sources (49%). Water isolates (86%) had a higher prevalence of the asal gene, which encodes for aggregation substance, whereas clinical (78%) and farm animal isolates (87%) had a higher prevalence of the esp gene, encoding a surface exposed protein. This study generates knowledge on the genetic diversity of E. faecalis with antibiotic resistance and virulence characteristics from various sources in Malaysia. (C) 2017 The Authors. Published by Elsevier Limited.