Molecular cloning and tissue mRNA levels of 15 genes involved in lipid metabolism in Synechogobius hasta
Molecular cloning and tissue mRNA levels of 15 genes involved in lipid metabolism in Synechogobius hasta
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聚鱼脂代谢15个基因的分子克隆及组织mRNA水平
DOI：
10.1002/ejlt.201400164
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发表时间：
2015-04-01
影响因子：
2.7
通讯作者：
Hu, Wei
中科院分区：
文献类型：
--
作者：
Chen, Qi-Liang;Luo, Zhi;Hu, Wei
脂质代谢的调节是复杂的，是目前广泛的研究领域。本研究从集鱼肝脏中克隆了15个参与脂质代谢的基因的部分序列，包括g6pd、6pgd、me、icdh、fas、accα、accβ、lpl、atgl、hsla、hslb、cpt 1a、srebp-1、pparα和pparγ。系统发育分析进一步鉴定了这些基因，并证实了S. hasta的分类和进化地位。所有基因的 mRNA 在肝脏、脾脏、肌肉、鳃、脑、肠和心脏中均被检测到，但水平不同。
实际应用：脂肪堆积过多、脂质代谢紊乱已成为水产养殖持续健康发展的严重问题。本研究有助于在分子水平上研究鱼类脂质代谢的调节。基因的组织表达谱增加了我们对其生理作用的理解。
本研究克隆并鉴定了聚鱼脂代谢相关的15个基因的部分序列，包括g6pd、6pgd、me、icdh、fas、accα、accβ、lpl、atgl、hsla、hslb、cpt 1a、srebp-1、pparα和pparγ。系统发育分析进一步鉴定了这些基因，并证实了S. hasta的分类和进化地位。所有基因的 mRNA 在肝脏、脾脏、肌肉、鳃、脑、肠和心脏中均被检测到，但水平各不相同。
The regulation of lipid metabolism is complex, and is currently an extensive area of research. In this study, the partial sequences of 15 genes involved in lipid metabolism were cloned from the liver of Synechogobius hasta, including g6pd, 6pgd, me, icdh, fas, accα, accβ, lpl, atgl, hsla, hslb, cpt 1a, srebp-1, pparα, and pparγ. Phylogenetic analysis further identified these genes, and confirmed the classification and evolutionary status of S. hasta. mRNA of all genes was detected in the liver, spleen, muscle, gill, brain, intestine, and heart, but at varying levels.
Practical applications: Excessive fat accumulation and disordered lipid metabolism have become serious problems in the sustainable and healthy development of aquaculture. The present study facilitates studies on the regulation of lipid metabolism at the molecular level in fish. The tissue expression profiles of genes increase our understanding of their physiological roles.
In this study, the partial sequences of 15 genes involved in lipid metabolism were cloned and characterized from Synechogobius hasta, including g6pd, 6pgd, me, icdh, fas, accα, accβ, lpl, atgl, hsla, hslb, cpt 1a, srebp-1, pparα, and pparγ. Phylogenetic analysis further identified these genes, and confirmed the classification and evolutionary status of S. hasta. mRNA of all genes was detected in the liver, spleen, muscle, gill, brain, intestine, and heart, but at the varying levels.