Aptamer photoregulation in vivo
Aptamer photoregulation in vivo
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DOI：
10.1073/pnas.1420105111
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发表时间：
2014-12-02
影响因子：
11.1
通讯作者：
Kohane, Daniel S.
中科院分区：
文献类型：
--
作者：
Li, Lele;Tong, Rong;Kohane, Daniel S.
适体作为治疗剂的体内应用可以通过增强靶特异性积累同时最小化脱靶摄取来改善。我们设计了一个光触发系统，允许时空调节的适体活性在体外和体内。通过将适体与光不稳定的互补寡核苷酸杂交来防止适体与细胞结合。在肿瘤部位照射后，适体被释放，导致延长的肿瘤内滞留。与游离适体相比，适体在肝脏和肾脏中的相对分布也显著降低。
The in vivo application of aptamers as therapeutics could be improved by enhancing target-specific accumulation while minimizing off-target uptake. We designed a light-triggered system that permits spatiotemporal regulation of aptamer activity in vitro and in vivo. Cell binding by the aptamer was prevented by hybridizing the aptamer to a photo-labile complementary oligonucleotide. Upon irradiation at the tumor site, the aptamer was liberated, leading to prolonged intratumoral retention. The relative distribution of the aptamer to the liver and kidney was also significantly decreased, compared to that of the free aptamer.