A pilot behavioural and neuroimaging investigation on photothrombotic stroke models in rhesus monkeys
A pilot behavioural and neuroimaging investigation on photothrombotic stroke models in rhesus monkeys
复制标题
恒河猴光血栓性中风模型的初步行为和神经影像学研究
DOI：
10.1016/j.jneumeth.2021.109291
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发表时间：
2021-07-27
影响因子：
3
通讯作者：
Wang，Jianhong
中科院分区：
文献类型：
--
作者：
Zhang，Zhiting;Wang，Shuguo;Wang，Jianhong
背景缺血性脑卒中可导致人类长期残疾，目前尚无有效的临床治疗方法。非人灵长类动物大脑中动脉闭塞（MCAO）模型已被证明是有价值的translational stroke research.New methodIn the current study，光血栓（PT）中风模型建立在恒河猴大脑中动脉（MCA）近端或远端段血栓形成。这项研究是第一个比较的两种方法PT中风的猴子使用的行为和生理测量和MRI scannes.ResultsThe实验发现，梗死发生在MCA的目标区域，与所有的猴子有受损的行为所反映的赤字在神经功能，和电机和认知的对象检索绕道（ORD）任务。大脑中动脉远端血栓形成的猴子伴随着大脑侧裂区、邻近中央前回和中央后回的连续光刺激，在PT卒中后第4天和第7天，在行为方面有类似的损害，并显示出小水肿体积和近端MCA血栓形成的趋势。刺激可以在恒河猴中提供一致和良好耐受的局灶性缺血，与其他PT中风模型相比，这些模型通常单独在动物的运动皮层进行，并具有时间效应。刺激性PT脑卒中模型是一种很有前途的研究人类脑卒中病理生理和开发新疗法的猕猴缺血性脑卒中模型。
BackgroundIschemic stroke leads to a long-term disability in humans and no efficient clinical therapy exists to date. The middle cerebral artery occlusion (MCAO) model in non-human primates has shown to be of value for translational stroke research.New methodIn the current study, a photothrombotic (PT) stroke model was established in rhesus monkeys with either a proximal or distal segment of middle cerebral artery (MCA) thrombosis. This study is the first that compares the two approaches of PT stroke in monkeys using behavioral and physiological measurements and MRI scans.ResultsThe experiment found that infarct occurred in the MCA target regions, with all monkeys having impaired behavior reflected by deficits in neurologic function, and motor and cognition in object retrieval detour (ORD) task. The monkeys with distal MCA thrombosis developed with sequential photo-irritations of the Sylvian fissure zone, adjacent central anterior gyrus and central posterior gyrus, had similar impairments with respect to behavior and showed a tendency of a small edema volume with proximal MCA thrombosis at days 4 and 7 post PT stroke.Comparison with existing methodsThe distal MCA thrombosis developed with sequential photo-irritations might provide a consistent and well-tolerated focal ischemia in rhesus monkeys, compared with other PT stroke models which usually were singly conducted on the animal’s motor cortex and had a temporal effect.ConclusionsThe sequentially photo-irritated PT stroke model is a promising ischemic stroke model in rhesus monkey for studying human stroke pathology and physiology and for new therapies development.