Extensive Hair Shaft Growth after Mouse Whisker Follicle Isolation, Cryopreservation and Transplantation in Nude Mice.
Extensive Hair Shaft Growth after Mouse Whisker Follicle Isolation, Cryopreservation and Transplantation in Nude Mice.
复制标题
DOI：
10.1371/journal.pone.0145997
复制
发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Hoffman RM
中科院分区：
文献类型：
--
作者：
Cao W;Li L;Tran B;Kajiura S;Amoh Y;Liu F;Hoffman RM
我们以前证明，整个毛囊可以冷冻保存，以保持其干细胞分化的潜力。在本研究中，我们证明了冷冻保存的小鼠须毛囊保持其毛发生长潜力。与甘油相比，DMSO能更好地冷冻保存小鼠须状卵泡。冷冻保存的毛囊也保持了毛囊相关多能（HAP）干细胞，证明了P75 NTR的表达。DMSO冷冻保存的毛囊在裸鼠皮下移植导致广泛的毛纤维生长超过8周，表明冷冻保存的毛囊的毛干生长的功能恢复。
We previously demonstrated that whole hair follicles could be cryopreserved to maintain their stem-cells differentation potential. In the present study, we demonstrated that cryopreserved mouse whisker hair follicles maintain their hair growth potential. DMSO better cryopreserved mouse whisker follicles compared to glycerol. Cryopreserved hair follicles also maintained the hair follicle-associated-pluripotent (HAP) stem cells, evidenced by P75NTR expression. Subcutaneous transplantation of DMSO-cryopreserved hair follicles in nude mice resulted in extensive hair fiber growth over 8 weeks, indicating the functional recovery of hair shaft growth of cryopreserved hair follicles.