Sugar metabolism during growth and development in sugarcane internodes
Sugar metabolism during growth and development in sugarcane internodes
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DOI：
10.2135/cropsci1999.0011183x0039000200030x
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发表时间：
1999-03-01
期刊：
影响因子：
2.3
通讯作者：
Lingle, SE
中科院分区：
文献类型：
--
作者：
Lingle, SE
甘蔗(Saccharum spp.)杂交种)具有较高蔗糖浓度的基因型是可取的。节间贮藏组织发育过程中蔗糖代谢酶的活性决定了总糖积累速率和最终蔗糖浓度。7个商品品种的节间在伸长前进行了标记，并在两个生长季节的7-12月间间隔取样。节间伸长大约完成380摄氏度(基础温度18摄氏度)。干物质积累到约800摄氏度。水分含量从约920克公斤(-1)鲜重(FW)降至约720克公斤(-1)。可溶性酸性转化酶和蔗糖合成酶(蔗糖裂解方向)在伸长过程中活性出现峰值，然后下降到较低水平。中性转化酶和蔗糖-磷酸合成酶活性在发育过程中呈上升趋势。随着节间的发育，蔗糖积累，蔗糖/总糖比值接近1.0，但多数节间蔗糖酶和蔗糖合成酶的联合蔗糖裂解活性大于蔗糖-磷酸合成酶的合成活性。糖分积累速率与测定的酶活性之间没有一致的相关性。节间发育过程中蔗糖含量与蔗糖磷酸合成酶活性以及蔗糖磷酸合成酶和酸性转化酶活性的差值相关。成熟节间蔗糖含量与任何酶活性均不相关。这些数据不支持蔗糖合成酶活性与蔗糖积累速率有关的假说，但支持蔗糖磷酸合成酶和酸性转化酶在甘蔗节间成熟过程中决定蔗糖浓度的假说。
Development of sugarcane (Saccharum spp. hybrids) genotypes with higher sucrose concentrations is desirable. Activities of sucrose-metabolizing enzymes during development of internodal storage tissue mag determine the rate of total sugar accumulation and final sucrose concentration. Internodes of seven commercial cultivars were tagged prior to elongation and sampled at intervals from July until December during two growing seasons. Internode elongation was completed by about 380 degrees C d (base temperature 18 degrees C). Dry matter accumulated until about 800 degrees C d. Water content decreased from about 920 to about 720 g kg(-1) fresh weight (FW). Soluble acid invertase and sucrose synthase (sucrose cleavage direction) had peaks of activity during elongation, then declined to lower levels. Neutral invertase and sucrose-phosphate synthase activities increased during development. Sugar accumulated, and the sucrose to total sugar ratio approached 1.0 as internodes developed, although the combined sucrose cleavage activity of the invertases and sucrose synthase in most internodes was greater than the synthetic activity of sucrose-phosphate synthase. Sugar accumulation rate was not consistently correlated with activity of My enzyme assayed. Sucrose content during internode development was correlated with sucrose-phosphate synthase activity and the difference between sucrose phosphate synthase and acid invertase activities. Sucrose content of mature internodes was not correlated with any enzyme activity. The data do not support the hypothesis that sucrose synthase activity is related to the rate of sugar accumulation, but do support the hypothesis that sucrose-phosphate synthase and acid invertase play key roles in determining sucrose concentration during maturation in sugarcane internodes.