Increased IκB Expression and Diminished Nuclear NF-κB in Human Mononuclear Cells following Hydrocortisone Injection
Increased IκB Expression and Diminished Nuclear NF-κB in Human Mononuclear Cells following Hydrocortisone Injection
复制标题
DOI：
10.1210/jcem.84.9.6104
复制
发表时间：
1999-09
期刊：
影响因子：
--
通讯作者：
A. Aljada;H. Ghanim;E. Assian;P. Mohanty;Wael Hamouda;R. Garg;P. Dandona
中科院分区：
文献类型：
--
作者：
A. Aljada;H. Ghanim;E. Assian;P. Mohanty;Wael Hamouda;R. Garg;P. Dandona
我们最近证明氢化可的松和其他糖皮质激素抑制单核细胞(MNC)和多形核白细胞(PMNL)产生的活性氧(ROS)。由于NF-κB/IκB系统调节促炎基因的转录，包括那些负责ROS产生的基因，我们在体内验证了氢化可的松可能刺激IκB的产生从而抑制NF-κB从胞浆转位到细胞核的假说。4名正常人静脉注射氢化可的松100毫克。分别于注射前、注射后1、2、4、8、24小时采集血样。用标准技术从单个核细胞中提取核提取液和总细胞裂解物。单核细胞匀浆中I-κB水平在1小时升高，2-4小时达到高峰，8小时开始下降，24小时恢复到基线水平。中性粒细胞核提取液中的NF-κB在1h开始下降，4h达到最低点，8h逐渐升高，并恢复到基础水平。
We have recently demonstrated that hydrocortisone and other glucocorticoids inhibit reactive oxygen species (ROS) generation by mononuclear (MNC) and polymorphonuclear leucocytes (PMNL). Since NF-κB/IκB system regulates the transcription of proinflammatory genes, including those responsible for ROS generation, we tested the hypothesis that hydrocortisone may stimulate IκB production thus inhibiting NF-κB translocation from the cytosol into the nucleus in MNC, in vivo. One hundred milligram of hydrocortisone was injected intravenously into 4 normal subjects. Blood samples were obtained prior to the injection and at 1, 2, 4, 8 and 24 hr after the injection. Nuclear extracts and total cell lysates were prepared from MNC by standard techniques. IκB levels in MNC homogenates increased at 1 hr, peaked at 2–4 hr, started to decrease at 8 hr, and returned to baseline levels at 24 hr. NF-κB in MNC nuclear extracts decreased at 1 hr, reached a nadir at 4 hr, gradually increased at 8 hr and returned back to baseline...