First Report of blaVIM-4- and mcr-9-Coharboring Enterobacter Species Isolated from a Pediatric Patient
First Report of blaVIM-4- and mcr-9-Coharboring Enterobacter Species Isolated from a Pediatric Patient
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DOI：
10.1128/msphere.00629-19
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发表时间：
2019-09-01
期刊：
影响因子：
4.8
通讯作者：
Kreiswirth, Barry N.
中科院分区：
文献类型：
--
作者：
Chavda, Kalyan D.;Westblade, Lars F.;Kreiswirth, Barry N.
从美国一家医院的儿科患者中回收了携带bla（Vim-4）和mcr-9的霍氏肠杆菌分离株。bla（Vim-4）和mcr-9基因携带在相同的IncH 12质粒pME-1a上。该分离株对粘菌素敏感，未观察到粘菌素抗性诱导。mcr-9基因位于两个插入元件IS 903和IS 1之间，但缺乏在携带mcr-9的其他分离株中发现的下游调控基因（qseC和qseB）。重要信息我们描述了从一名有埃及旅行史的美国儿科患者中回收的携带bla（Vim-4）和mcr-9的临床肠杆菌分离株的完整基因组组装和序列。此外，据我们所知，这是美国首次报告同时携带bla（Vim-4）和mcr-9的肠杆菌属分离株。bla（Vim-4）和mcr-9基因携带在相同的IncH 12质粒pME-1a上。该分离株对粘菌素敏感，未观察到粘菌素抗性诱导。mcr-9基因位于两个插入元件（IS 903和IS 1）之间，但缺乏在携带mcr-9的其他分离株中发现的下游调控基因（qseC和qseB）。
An Enterobacter hormaechei isolate harboring bla(VIM-4) and mcr-9 was recovered from a pediatric patient in a U.S. hospital. The bla(VIM-4) and mcr-9 genes are carried on the same IncH12 plasmid, pME-1a. The isolate tested susceptible to colistin, without observed induction of colistin resistance. The mcr-9 gene is located between two insertion elements, IS903 and IS1, but lacks the downstream regulatory genes (qseC and qseB) found in other isolates that harbor mcr-9.IMPORTANCE We describe the complete genome assembly and sequence of a clinical Enterobacter isolate harboring both bla(VIM-4) and mcr-9 recovered from a pediatric patient in the United States with a history of travel to Egypt. Moreover, to the best of our knowledge, this is the first report of an Enterobacter isolate harboring both bla(VIM-4) and mcr-9 from the United States. The bla(VIM-4) and mcr-9 genes are carried on the same IncH12 plasmid, pME-1a. The isolate tested susceptible to colistin, without observed induction of colistin resistance. The mcr-9 gene is located between two insertion elements, IS903 and IS1, but lacks the downstream regulatory genes (qseC and qseB) found in other isolates that harbor mcr-9.