Two coexisting pseudo-mirror heteromolecular telomeric G-quadruplexes in opposite loop progressions differentially recognized by a low equivalent of Thioflavin T.
Two coexisting pseudo-mirror heteromolecular telomeric G-quadruplexes in opposite loop progressions differentially recognized by a low equivalent of Thioflavin T.
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两个共存的伪镜像异分子端粒 G 四链体在相反的环进程中被低当量的硫黄素 T 差异识别
DOI：
10.1093/nar/gkab755
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发表时间：
2021-10-11
影响因子：
14.9
通讯作者：
Zhang N
中科院分区：
文献类型：
--
作者：
Fu W;Jing H;Xu X;Xu S;Wang T;Hu W;Li H;Zhang N
端粒DNA G-突出端的最后3′-末端残基本质上不太精确。在这里，我们描述了最后3′-末端碱基的改变如何影响人类端粒DNA的两个不同的富含G的寡聚体之间的相互识别，形成异分子G-四链体（hetero-GQs）。在Na+溶液中，人端粒DNA的三个和单个重复片段，靶d（GGGTTAGGGTTAGGG）和探针d（TAGGGT）之间的关联产生两种共存形式的（3 + 1）杂合异源GQ：动力学上有利的LLP形式（左环进展）和药物控制的RLP形式（右环进展）。然而，先前仅报道了在相同探针d（TAGGGT）和具有一个额外3′-末端胸腺嘧啶的靶变体d（GGGTTAGGGTTAGGGT）之间采用单个LLP形式。此外，短的富含G的探针变体的侧翼碱基改变也显著影响杂-GQ的环进展。虽然看似两个伪镜计数器的合作伙伴，RLP-形式表现出的LLP-形式的偏好被识别的低当量的荧光染料硫磺素T（ThT）。在更大程度上，ThT优先结合RLP异源GQ比与相应的端粒DNA双链体上下文或其他几个代表性的单分子GQ。
The final 3′-terminal residue of the telomeric DNA G-overhang is inherently less precise. Here, we describe how alteration of the last 3′-terminal base affects the mutual recognition between two different G-rich oligomers of human telomeric DNA in the formation of heteromolecular G-quadruplexes (hetero-GQs). Associations between three- and single-repeat fragments of human telomeric DNA, target d(GGGTTAGGGTTAGGG) and probe d(TAGGGT), in Na+ solution yield two coexisting forms of (3 + 1) hybrid hetero-GQs: the kinetically favourable LLP-form (left loop progression) and the thermodynamically controlled RLP-form (right loop progression). However, only the adoption of a single LLP-form has been previously reported between the same probe d(TAGGGT) and a target variant d(GGGTTAGGGTTAGGGT) having one extra 3′-end thymine. Moreover, the flanking base alterations of short G-rich probe variants also significantly affect the loop progressions of hetero-GQs. Although seemingly two pseudo-mirror counter partners, the RLP-form exhibits a preference over the LLP-form to be recognized by a low equivalent of fluorescence dye thioflavin T (ThT). To a greater extent, ThT preferentially binds to RLP hetero-GQ than with the corresponding telomeric DNA duplex context or several other representative unimolecular GQs.
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DOI：
10.3390/molecules181012368
发表时间：
2013-10-08
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Bidzinska J;Cimino-Reale G;Zaffaroni N;Folini M
通讯作者：
Folini M
影响因子：
14.9
作者：
Brcic J;Plavec J
通讯作者：
Plavec J
影响因子：
15
作者：
Clore, GM;Kuszewski, J
通讯作者：
Kuszewski, J
影响因子：
14.9
作者：
Ambrus A;Chen D;Dai J;Bialis T;Jones RA;Yang D
通讯作者：
Yang D
影响因子：
48
作者：
Ivani I;Dans PD;Noy A;Pérez A;Faustino I;Hospital A;Walther J;Andrio P;Goñi R;Balaceanu A;Portella G;Battistini F;Gelpí JL;González C;Vendruscolo M;Laughton CA;Harris SA;Case DA;Orozco M
通讯作者：
Orozco M