Gold nanoparticle cellular uptake, toxicity and radiosensitisation in hypoxic conditions
Gold nanoparticle cellular uptake, toxicity and radiosensitisation in hypoxic conditions
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DOI：
10.1016/j.radonc.2013.12.013
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发表时间：
2014-02-01
影响因子：
5.7
通讯作者：
O'Sullivan, Joe M.
中科院分区：
文献类型：
--
作者：
Jain, Suneil;Coulter, Jonathan A.;O'Sullivan, Joe M.
背景与目的：金纳米粒子（GNP）是一种新型的放射增敏剂，在体内外均表现出放射增敏作用。肿瘤缺氧与癌症患者的放射抵抗和生存率降低有关。材料与方法：在体外研究了GNP在有氧和低氧条件下的摄取、定位、毒性和放射增敏作用。结果：GNP在低氧条件下的细胞摄取明显低于有氧条件。观察到暴露于GNP的缺氧MDA-MB-231乳腺癌细胞中细胞增殖的显著减少。在这些细胞中，显着的放射增敏发生在常氧和中度缺氧。然而，在近缺氧没有显着的sensitisation happened.Conclusions：GNP摄取发生在缺氧条件下，导致中度，但不是极端缺氧的乳腺癌细胞系的放射增敏。这些发现对于开发用于癌症治疗的GNP可能是重要的。(C)2014爱思唯尔爱尔兰有限公司版权所有。
Background and purpose: Gold nanoparticles (GNPs) are novel agents that have been shown to cause radiosensitisation in vitro and in vivo. Tumour hypoxia is associated with radiation resistance and reduced survival in cancer patients. The interaction of GNPs with cells in hypoxia is explored.Materials and methods: GNP uptake, localization, toxicity and radiosensitisation were assessed in vitro under oxic and hypoxic conditions.Results: GNP cellular uptake was significantly lower under hypoxic than oxic conditions. A significant reduction in cell proliferation in hypoxic MDA-MB-231 breast cancer cells exposed to GNPs was observed. In these cells significant radiosensitisation occurred in normoxia and moderate hypoxia. However, in near anoxia no significant sensitisation occurred.Conclusions: GNP uptake occurred in hypoxic conditions, causing radiosensitisation in moderate, but not extreme hypoxia in a breast cancer cell line. These findings may be important for the development of GNPs for cancer therapy. (C) 2014 Elsevier Ireland Ltd. All rights reserved.