Integrin-mediated preadipocyte adhesion and migration on laminin-1
Integrin-mediated preadipocyte adhesion and migration on laminin-1
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DOI：
10.1114/1.1566446
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发表时间：
2003-05-01
影响因子：
3.8
通讯作者：
Wu, XM
中科院分区：
文献类型：
--
作者：
Patrick, CW;Wu, XM
细胞粘附和迁移是许多生物学过程中的关键事件，并且依赖于细胞外基质蛋白。了解这些细胞在脂肪形成的事件是至关重要的，阐明生物学机制的前脂肪细胞的具体方面的肥胖症，糖尿病和脂肪组织的发展，药物筛选和组织工程策略的设计。我们定量研究了前脂肪细胞在层粘连蛋白-1表面的粘附和迁移，并确定了层粘连蛋白-1的候选同源前脂肪细胞受体。在粘附研究中，我们发现，前脂肪细胞容易粘附层粘连蛋白-1相比，其他细胞外基质蛋白。此外，免疫细胞化学分析表明，阵列的整合素分子存在于前脂肪细胞的表面。使用沉降粘附试验定量评估前脂肪细胞对层粘连蛋白-1的粘附，结果表明前脂肪细胞对层粘连蛋白-1的粘附是由α 1 β 1整联蛋白介导的。此外，数字延时显微镜和定量细胞跟踪显示，α（1）β（1）整合素的抑制导致层粘连蛋白-1表面的前脂肪细胞迁移的废除。这些结果强烈支持这一假设，即前脂肪细胞的粘附和迁移层粘连蛋白-1基质的调节，部分，整合素。(C)2003生物医学工程学会。
Cell adhesion and migration are key events in many biological processes and depend on extracellular matrix proteins. Understanding these cellular events in adipogenesis is paramount to elucidating the biological mechanisms underlying preadipocyte-specific aspects of obesity, diabetes, and adipose tissue development for the design of pharmaceutical screening and tissue engineering strategies. We quantitatively investigated preadipocyte adhesion and migration on laminin-1 surfaces and identified candidate cognate preadipocyte receptors for laminin-1. In adhesion studies, we found that preadipocytes readily adhered to laminin-1 as compared with other extracellular matrix proteins. In addition, immunocytochemical analysis demonstrated that an array of integrin molecules was present on the surface of preadipocytes. Preadipocyte adhesion on laminin-1 was quantitatively assessed using a sedimentation adhesion assay, and results suggested that preadipocyte adhesion to laminin-1 was mediated by the alpha(1)beta(1) integrin. In addition, digital time-lapse microscopy and quantitative cell tracking revealed that inhibition of the alpha(1)beta(1) integrin resulted in abrogation of preadipocyte migration on laminin-1 surfaces. These results strongly support the hypothesis that preadipocyte adhesion to and migration on laminin-1 substrata are regulated, in part, by integrins. (C) 2003 Biomedical Engineering Society.