Synthesis, characterization, and cytocompatibility of efastomeric, biodegradable poly(ester-urethane)ureas based on poly(caprolactone) and putrescine
Synthesis, characterization, and cytocompatibility of efastomeric, biodegradable poly(ester-urethane)ureas based on poly(caprolactone) and putrescine
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DOI：
10.1002/jbm.10204
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发表时间：
2002-09-05
期刊：
影响因子：
--
通讯作者：
Wagner, WR
中科院分区：
文献类型：
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作者：
Guan, JJ;Sacks, MS;Wagner, WR
在心血管系统中用于对力学性能有要求的应用的组织工程，可能需要在细胞 - 支架构建体植入之前对其进行力学调节。适合这种应用的支架特性包括高弹性和强度，以及可控的生物降解和细胞黏附特性。为了满足这些设计标准，我们从聚己内酯和1,4 - 二异氰酸丁烷合成了一系列聚（酯 - 氨酯）脲（PEUUs）。赖氨酸乙酯（Lys）或腐胺被用作扩链剂。为了促进细胞黏附，先用射频辉光放电对PEUUs进行表面改性，然后偶联精氨酸 - 甘氨酸 - 天冬氨酸 - 丝氨酸（RGDS）。合成的PEUUs具有高度的柔韧性，断裂应变在660 - 895%之间，拉伸强度在9.2 - 29 MPa之间。在水缓冲液中孵育8周导致质量损失，从>50%（Lys扩链剂）到10%（腐胺扩链剂）不等。用含有来自使用Lys或腐胺扩链剂的PEUUs降解产物的培养基培养4天的人内皮细胞没有显示出毒性作用。对于未改性的PEUU表面，细胞黏附是在组织培养聚苯乙烯上测量值的85%（p < 0.01），而在RGDS改性的PEUUs上是聚苯乙烯的>160%（p < 0.001）。这些可生物降解的PEUUs显示出未来在心血管组织工程或其他软组织应用中作为细胞支架的应用潜力。（C）2002威利期刊公司
The engineering of tissue for mechanically demanding applications in the cardiovascular system is likely to require mechanical conditioning of cell-scaffold constructs prior to their implantation. Scaffold properties amenable to such an application include high elasticity and strength coupled with controllable biodegradative and cell-adhesive properties. To fulfill such design criteria, we have synthesized a family of poly(ester-urethane)ureas (PEUUs) from polycaprolactone and 1,4-diisocyanatobutane. Lysine ethyl ester (Lys) or putrescine was used as chain extenders. To encourage cell adhesion, PEUUs were surface modified with radio-frequency glow discharge followed by coupling of Arg-Gly-Asp-Ser (RGDS). The synthesized PELTUs were highly flexible, with breaking strains of 660-895% and tensile strengths from 9.2-29 MPa. Incubation in aqueous buffer for 8 weeks resulted in mass loss, from >50% (Lys chain extender) to 10% (putrescine chain extender). Human endothelial cells cultured for 4 days with medium containing the degradation products from PELTUs with either the Lys or putrescine chain extender showed no toxic effects. Cell adhesion was 85% of that measured on tissue-culture polystyrene for unmodified PEUU surfaces (p < 0.01) and > 160% (p < 0.001) of polystyrene on RGDS-modified PELTUs. These biodegradable PEUUs demonstrate potential for future application as cell scaffolds in cardiovascular tissue-engineering or other soft-tissue applications. (C) 2002 Wiley Periodicals, Inc.