Droplet Microfluidics for High-Resolution Virology.
Droplet Microfluidics for High-Resolution Virology.
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DOI：
10.1021/acs.analchem.2c00615
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发表时间：
2022-05
影响因子：
7.4
通讯作者：
Wenyang Jing;Hee-Sun Han
中科院分区：
文献类型：
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作者：
Wenyang Jing;Hee-Sun Han
微流控技术由于其小的长度尺度、并行化以及各种分析和驱动功能的模块化，已经开启了细胞和分子测定的新时代。特别是液滴微流体技术，它能够快速、可重复地产生充当单个反应器的液滴，从而为生物学提供了新的工具。这项技术的一个显著受益者是单细胞RNA测序，它揭示了生命基本单位的新的异质性和相互作用。然而，病毒远远超过细胞生命的多样性，影响所有生态系统的动态，是全球健康危机的长期来源。尽管它们对世界产生了影响，但高通量和高分辨率的病毒谱分析一直很困难，传统方法仅限于群体水平的平均值、大样本量和很少的可培养宿主。因此，大多数病毒尚未被识别和研究。液滴微流体技术有可能解决许多这些限制，并为病毒学提供新的灵敏度和通量水平。该功能突出了最近将液滴微流体技术应用于病毒检测和研究的努力，包括诊断，病毒-宿主相互作用和细胞非依赖性病毒测定。与传统的病毒学方法相结合，液滴微流体技术应该被证明是一种有效的工具，可以更好地了解地球上最丰富的生物物种。
Microfluidics has enabled a new era of cellular and molecular assays due to the small length scales, parallelization, and the modularity of various analysis and actuation functions. Droplet microfluidics, in particular, has been instrumental in providing new tools for biology with its ability to quickly and reproducibly generate drops that act as individual reactors. A notable beneficiary of this technology has been single-cell RNA sequencing, which has revealed new heterogeneities and interactions for the fundamental unit of life. However, viruses far surpass the diversity of cellular life, affect the dynamics of all ecosystems, and are a chronic source of global health crises. Despite their impact on the world, high-throughput and high-resolution viral profiling has been difficult, with conventional methods being limited to population-level averaging, large sample volumes, and few cultivable hosts. Consequently, most viruses have not been identified and studied. Droplet microfluidics holds the potential to address many of these limitations and offers new levels of sensitivity and throughput for virology. This Feature highlights recent efforts that have applied droplet microfluidics to the detection and study of viruses, including for diagnostics, virus-host interactions, and cell-independent virus assays. In combination with traditional virology methods, droplet microfluidics should prove a potent tool toward achieving a better understanding of the most abundant biological species on Earth.