Developmentally regulated expression of Drosophila chorion genes introduced at diverse chromosomal positions.
Developmentally regulated expression of Drosophila chorion genes introduced at diverse chromosomal positions.
复制标题
在不同染色体位置引入的果蝇绒毛膜基因的发育调节表达。
DOI：
10.1016/0022-2836(86)90404-3
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发表时间：
1986
影响因子：
5.6
通讯作者：
A. Spradling
中科院分区：
文献类型：
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作者：
B. Wakimoto;L. Kalfayan;A. Spradling
果蝇绒毛膜基因被组织成两个簇，在卵泡细胞中选择性扩增。在卵子发生过程中，单个基因的转录受到时间调控，导致绒毛膜mRNA积累的不同阶段特异性特征。利用P元件介导的基因转移研究了编码主要绒毛膜蛋白s15-1和s38-1的基因的调控。转化绒毛膜基因整合在不同的染色体位置表现出适当的组织特异性和阶段特异性表达，尽管从基因簇分离。定性正常表达不依赖于插入的DNA进行扩增的能力。然而，染色体位置定量影响转化基因产生的RNA。在校正由于一些插入序列的扩增而导致的基因剂量差异后，单个转化基因产生的每个基因拷贝的RNA水平变化约10倍。用ans 38 -1-lacZ融合基因进行的转化实验表明，足够用于s38 - 1表达的阶段特异性程序的顺式调控序列被限制在相对于38 - 1起始位点的-748和+573之间的1.3 × 103个碱基对的片段内。最后，在两个38 - 1插入位点诱导卵室特异性扩增，表明扩增控制元件位于该基因附近。
Drosophilachorion genes are organized into two clusters that are selectively amplified in the ovarian follicle cells. During oogenesis the transcription of individual genes is temporally regulated, resulting in distinct, stage-specific profiles of chorion mRNA accumulation. P element-mediated gene transfer was used to study the regulation of genes encoding the major chorion proteins s15-1 and s38-1. Transformed chorion genes integrated at diverse chromosomal locations exhibited proper tissue-specific and stage-specific expression, despite separation from the gene clusters. Qualitatively normal expression was not dependent on the ability of the inserted DNA to undergo amplification. However, chromosome position quantitatively influenced the RNA produced by the transformed genes. The level of RNA per gene copy produced by individual transformed genes varied approximately tenfold, after correction for differences in gene dosage due to the amplification of some inserted sequences. Transformation experiments with ans38-1-lacZfusion gene demonstrated thatcis-regulatory sequences sufficient for the stage-specific program ofs38-1expression were confined to a 1.3 × 103base-pair segment between −748 and +573 relative to thes38-1initiation site. Finally, egg chamber-specific amplification was induced at the site of twos38-1insertions, suggesting that an amplification control element resides near this gene.
β-半乳糖苷酶基因融合体用于分析大肠杆菌和酵母中的基因表达。
影响因子：
--
作者：
Casadaban,MJ;Martinez-Arias,A;Shapira,SK;Chou,J
通讯作者：
Chou,J