Dynamics of appetite-mediated gene expression in daidzein-fed female rats in the meal-feeding method
Dynamics of appetite-mediated gene expression in daidzein-fed female rats in the meal-feeding method
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DOI：
10.1080/09168451.2015.1025034
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发表时间：
2015-08-03
影响因子：
1.6
通讯作者：
Kishida, Taro
中科院分区：
文献类型：
--
作者：
Fujitani, Mina;Mizushige, Takafumi;Kishida, Taro
我们之前发现大豆苷元减少了雌性大鼠的食物摄入量。本研究旨在阐明食欲介导的神经肽的动力学与大豆黄酮的食欲减退作用之间的关系。我们检查了每日三餐喂养方法期间下丘脑和小肠中食欲介导的基因表达。大豆苷元特别在第二次喂食时具有抑制食欲的作用。对照组喂食后下丘脑中的神经肽-Y (NPY) 和甘丙肽 mRNA 水平显着升高，但大豆黄酮组则没有，表明大豆黄酮减弱了餐后 NPY 和甘丙肽表达的增加。大豆苷元组在进食后下丘脑中促肾上腺皮质激素释放激素（CRH）mRNA水平较高，小肠中胆囊收缩素（CCK）mRNA水平增加，表明CCK参与了这种厌食效应的下丘脑调节。因此，黄豆苷元可能通过抑制下丘脑中NPY和甘丙肽的表达并增加CRH的表达来诱发厌食。
We previously found that daidzein decreased food intake in female rats. The present study aimed to elucidate the relationship between dynamics of appetite-mediated neuropeptides and the anorectic effect of daidzein. We examined appetite-mediated gene expression in the hypothalamus and small intestine during the 3 meals per day feeding method. Daidzein had an anorectic effect specifically at the second feeding. Neuropeptide-Y (NPY) and galanin mRNA levels in the hypothalamus were significantly higher after feeding in the control but not in the daidzein group, suggesting that daidzein attenuated the postprandial increase in NPY and galanin expression. The daidzein group had higher corticotrophin-releasing hormone (CRH) mRNA levels in the hypothalamus after feeding, and increased cholelcystokinin (CCK) mRNA levels in the small intestine, suggesting that CCK is involved in the hypothalamic regulation of thisanorectic effect. Therefore, daidzein may induce anorexia by suppressing expression of NPY and galanin and increasing expression of CRH in the hypothalamus.