A NOVEL METHOD OF PREPARING RAT-MONOCLONAL ANTIBODY-PRODUCING HYBRIDOMAS BY USING RAT MEDIAL ILIAC LYMPH-NODE CELLS
A NOVEL METHOD OF PREPARING RAT-MONOCLONAL ANTIBODY-PRODUCING HYBRIDOMAS BY USING RAT MEDIAL ILIAC LYMPH-NODE CELLS
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DOI：
10.1247/csf.20.151
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发表时间：
1995-04-01
影响因子：
1.5
通讯作者：
SADO, Y
中科院分区：
文献类型：
--
作者：
KISHIRO, Y;KAGAWA, M;SADO, Y
建立了一种制备产生大鼠单克隆抗体的杂交瘤的新方法。将来自大鼠的增大的内侧髂淋巴结通过后足垫注射抗原和弗氏佐剂的乳液用于细胞融合。使用卵清蛋白作为代表性抗原。用这种方法产生目的抗体的杂交瘤的发生率比用小鼠或大鼠脾细胞的常规方法产生的杂交瘤高约10倍。产生IgG1、IgG2a、IgG2b和IgG2c的杂交瘤的平均百分比分别为37.1%、47.0%、15.9%和0.0%。在该方法中，单次注射抗原就足以进行免疫。
A novel method of preparing hybridomas producing rat monoclonal antibodies was established. The enlarged medial iliac lymph nodes from rats injected via hind footpads with an emulsion of antigen and Freund's adjuvant were used for cell fusion. Ovalbumin was used as a representative antigen. The incidence of hybridomas producing antibody of interest with this method was about 10 times higher than that of hybridomas with the conventional method using mouse or rat spleen cells. The average percentages of hybridomas producing IgG1, IgG2a, IgG2b and IgG2c were 37.1%, 47.0%, 15.9% and 0.0%, respectively. A single injection with antigen was sufficient for immunization in this method.