Exogenous nitric oxide protect cucumber roots against oxidative stress induced by salt stress
Exogenous nitric oxide protect cucumber roots against oxidative stress induced by salt stress
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DOI：
10.1016/j.plaphy.2007.05.005
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发表时间：
2007-08-01
影响因子：
6.5
通讯作者：
Wei, Min
中科院分区：
文献类型：
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作者：
Shi, Qinghua;Ding, Fei;Wei, Min
线粒体是亚细胞的细胞器，具有基本上氧化型的代谢。其中活性氧（ROS）的产生在应激条件下增加，并引起氧化损伤。以黄瓜幼苗为材料，研究了外源一氧化氮（NO）供体硝普钠（SNP）对100 mM NaCl胁迫下线粒体活性氧代谢、质膜和液泡膜功能的影响。NaCl处理引起黄瓜根系线粒体H_2O_2的大量积累，导致线粒体膜脂过氧化严重; 50 μ M SNP处理可刺激ROS清除酶活性，降低NaCl诱导的黄瓜根系线粒体H_2O_2的积累。结果，线粒体脂质过氧化反应减少。进一步的研究表明，SNP的应用减轻了NaCl对质膜和/或液泡膜H+-ATPase和H+-PPase的抑制。而亚铁氰化钠（SNP的类似物，不释放NO）的应用没有显示SNP的效果，此外，SNP的效果通过添加血红蛋白（NO清除剂）而逆转。(c)2007年，Elsevier Masson SAS。All rights reserved.
Mitochondria are subcellular organelles with an essentially oxidative type of metabolism. The production of reactive oxygen species (ROS) in it increases under stress conditions and causes oxidative damage. In the present study, effects of exogenous sodium nitroprusside (SNP), a nitric oxide (NO) donor, on both the ROS metabolism in mitochondria and functions of plasma membrane (PM) and tonoplast were studied in cucumber seedlings treated with 100 mM NaCl. NaCl treatment induced significant accumulation Of H2O2 and led to serious lipid peroxidation in cucumber mitochondria, and the application of 50 mu M SNP stimulated ROS-scavenging enzymes and reduced accumulation of H2O2 in mitochondria of cucumber roots induced by NaCl. As a result, lipid peroxidation of mitochondria decreased. Further investigation showed that application of SNP alleviated the inhibition of H+-ATPase and H+-PPase in PM and/or tonoplast by NaCl. While application of sodium ferrocyanide (an analog of SNP that does not release NO) did not show the effect of SNP, furthermore, the effects of SNP were reverted by addition of hemoglobin (a NO scavenger). (c) 2007 Elsevier Masson SAS. All rights reserved.