GENETIC-TRANSFORMATION OF THE LYME-DISEASE AGENT BORRELIA-BURGDORFERI WITH COUMARIN-RESISTANT GYRB
GENETIC-TRANSFORMATION OF THE LYME-DISEASE AGENT BORRELIA-BURGDORFERI WITH COUMARIN-RESISTANT GYRB
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DOI：
10.1128/jb.176.19.6045-6049.1994
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发表时间：
1994-10-01
影响因子：
3.2
通讯作者：
GARON, CF
中科院分区：
文献类型：
--
作者：
SAMUELS, DS;MACH, KE;GARON, CF
没有有用的方法来遗传操纵伯氏疏螺旋体，莱姆病的病原体，以前已经开发。我们已经使用了对香豆素抗生素香豆素霉素A（1）的抗性作为遗传标记来监测B的转化，香豆素霉素A是DNA促旋酶的抑制剂。burgdorferi）。将定点突变引入促旋酶B基因证明转化成功，提供了染色体上发生同源重组的证据，并确定DNA促旋酶B的Arg-133突变赋予B对香豆霉素A（1）的耐药性。burgdorferi。香豆霉素A（1）-抗性gyrB标记和遗传转化现在可以应用于解剖生理和莱姆病病原体的分子遗传水平上的发病机制。
No useful method to genetically manipulate Borrelia burgdorferi, the causative agent of Lyme disease, has been developed previously. We have used resistance to the coumarin antibiotic coumermycin A(1), an inhibitor of DNA gyrase, as a genetic marker to monitor the transformation of B. burgdorferi by electroporation. Introduction of site directed mutations into the gyrB gene demonstrated that transformation was successful, provided evidence that homologous recombination occurs on the chromosome, and established that mutations at Arg-133 of DNA gyrase B confer coumermycin A(1) resistance in B. burgdorferi. The coumermycin A(1)-resistant gyrB marker and genetic transformation can now be applied toward dissecting the physiology and pathogenesis of the Lyme disease agent on a molecular genetic level.