[Electroacupuncture at "Zusanli"(ST36) and "Xuanzhong"(GB39) improves inflammatory reactions and reduces levels of serum MMP-3 and MMP-9 in adjuvant arthritis rats].
[Electroacupuncture at "Zusanli"(ST36) and "Xuanzhong"(GB39) improves inflammatory reactions and reduces levels of serum MMP-3 and MMP-9 in adjuvant arthritis rats].
复制标题
DOI：
10.13702/j.1000-0607.180555
复制
发表时间：
2019-09-25
期刊：
影响因子：
--
通讯作者：
Zhu, Jun
中科院分区：
文献类型：
--
作者：
Chen, Yu-Zhou;Su, Cheng-Guo;Zhu, Jun
目的：观察电针“足三里”、“悬钟”对佐剂性关节炎（AA）大鼠关节炎性反应及血清基质金属蛋白酶3（MMP-3）、9（MMP-9）含量的影响，探讨电针改善AA的作用机制。将40只雄性SD大鼠随机分为对照组、模型组、穴位组和非穴位组，每组10只。双侧足垫皮下注射完全弗氏佐剂（0.1mL）建立关节炎模型。电针（2 Hz，3V）于双侧ST 36、GB 39或两个非穴位（ST 36、GB 39左侧5 mm处）15 min，隔日1次，共8次。根据踝关节、跖关节、趾关节和足掌关节局部红斑和肿胀的严重程度进行关节炎指数评分（0-4分）。HE染色观察踝关节炎症情况。结果：模型组大鼠关节炎指数评分及血清MMP-3、MMP-9含量均较对照组明显升高（P
OBJECTIVE: To observe the effect of electroacupuncture (EA) at "Zusanli" (ST36) and "Xuanzhong" (GB39) on joint inflammatory reactions and serum matrix metalloproteinase-3 (MMP-3) and MMP-9 contents in rats with adjuvant arthritis (AA), so as to explore its mechanism underlying improvement of AA.METHODS: Forty male SD rats were randomly divided into control, model, acupoint and non-acupoint groups (n=10 in each group). The arthritis model was established by hypodermic injection of complete Freund's adjuvant (0.1 mL) into the bilateral footpads. EA (2 Hz, 3 V) was applied to bilateral ST36 and GB39 or two non-acupoints (5 mm left to ST36 and GB39) for 15 min, once every other day for a total of 8 times. The arthritis index score was evaluated according to the severity of local erythema and swelling of the ankle joint, plantar joint, toe joint and foot metacarpal joint (0-4 points). The inflammatory conditions of the ankle joint were observed by H.E. staining, and the contents of serum MMP-3 and MMP-9 were assayed by ELISA.RESULTS: The arthritis index score and serum concentrations of MMP-3 and MMP-9 were significantly increased in the model group relevant to the control group (P