CHRYSANTHEMUM STUNT VIROID - PRIMARY SEQUENCE AND SECONDARY STRUCTURE
CHRYSANTHEMUM STUNT VIROID - PRIMARY SEQUENCE AND SECONDARY STRUCTURE
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DOI：
10.1093/nar/9.12.2741
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发表时间：
1981-01-01
影响因子：
14.9
通讯作者：
SYMONS, RH
中科院分区：
文献类型：
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作者：
HASELOFF, J;SYMONS, RH
测定了菊花矮化类病毒（CSV）的356个核苷酸残基的序列。通过部分核糖核酸酶消化获得重叠的线性类病毒片段，在其5′-末端进行体外放射性标记，并用部分酶切法进行测序。在CSV序列中，69%包含在已发表的马铃薯纺锤块茎类病毒（PSTV）序列中。CSV和PSTV一级序列的差异表明，这两种类病毒的正链和推定的负链都不编码功能性多肽产物。然而，两种类病毒可以形成相似的二级结构，暗示类病毒结构在复制中的作用。
The sequence of the 356 nucleotide residues of chrysanthemum stunt viroid (CSV) has been determined. Overlapping linear viroid fragments were obtained by partial ribonuclease digestion, radiolabelledin vitroat their 5′-ends, and sequenced using partial enzymic cleavage methods. Of the CSV sequence, 69% is contained in the published sequence of potato spindle tuber viroid (PSTV). Differences in the primary sequence of CSV and PSTV suggest that neither the positive nor putative negative strands of these two viroids code for functional polypeptide products. However, the two viroids can form similar secondary structures, implicating a role for viroid structure in replication.