Immunogenicity and immunomodulatory effects of amnion-derived multipotent progenitor cells
Immunogenicity and immunomodulatory effects of amnion-derived multipotent progenitor cells
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DOI：
10.1016/j.humimm.2008.04.007
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发表时间：
2008-06-01
期刊：
影响因子：
2.7
通讯作者：
Zeevi, Adriana
中科院分区：
文献类型：
--
作者：
Banas, Richard Allan;Trumpower, Catherine;Zeevi, Adriana
这是第一个研究的临床相关人口的细胞来源于羊膜的人胎盘的免疫学特性。与来自羊膜的其他细胞不同，这些来自人羊膜的羊膜衍生的多能祖细胞（AMP细胞）在无血清条件下生长，并且从未在含有动物衍生组分的培养基存在下培养。本研究报道了AMP细胞的免疫学特性及其作为免疫调节剂的作用。AMP细胞的表征显示存在主要组织相容性复合物（MHC）I类，但缺乏II类抗原和缺乏共刺激分子B7-1和B7-2。非经典人类白细胞抗原（HLA）-G在培养的AMP细胞上以两种水平表达。干扰素-γ（IFN-γ）治疗后表达显著增加。培养的外周血单个核细胞对经辐照的AMP细胞没有反应，这表明通过标准混合淋巴细胞反应测量缺乏增殖。用IFN-γ培养AMP细胞没有逆转这一结果，也没有上调II类表达。AMP细胞被证明具有免疫调节能力，通过抑制外周血单核细胞增殖反应的有丝分裂原，同种异体抗原，和回忆抗原，但AMP细胞不能抑制预活化的T细胞母细胞对生长因子培养基的反应。AMP细胞的这种免疫调节作用被发现依赖于细胞与细胞的接触。(c)2008年美国组织相容性和免疫遗传学学会。爱思唯尔公司出版All rights reserved.
This is the first study on the immunologic properties of a clinically relevant population of cells derived from the amnion of human placenta. Unlike other cells from the amnion, these amnion-derived multipotent progenitor cells (AMP cells), from human amnion, grow in serum-free conditions and have never been cultured in the presence of medium containing animal-derived components. This study reports the immunologic characteristics of AMP cells and their roles as immunomodutators. Characterization of AMP cells revealed the presence of major histocompatibitity complex (MHC) class I but the lack of class II antigens and absence of co-stimulatory molecules B7-1 and B7-2. The nonclassical human leukocyte antigen (HLA)-G was expressed at tow levels on cultured AMP cells. Expression was significantly increased after interferon-gamma (IFN-gamma) treatment. Cultured peripheral blood mononuclear cells did not respond to irradiated AMP cells, indicated by lack of proliferation as measured by standard mixed lymphocyte reaction. Culturing AMP cells with IFN-gamma did not reverse this result and did not upregulate class II expression. The AMP cells were shown to have immunomodulatory capabilities by inhibiting peripheral blood mononuclear cell proliferative responses to mitogen, alloantigen, and recall antigen, but the AMP cells were unable to inhibit preactivated T-cell blast response to growth factor media. This immunomodulatory effect of AMP cells was found to be dependent on cell-to-cell contact. (c) 2008 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved.