Components of the mitogen-activated protein kinase cascade are activated in hepatic cells by Echinococcus multilocularis metacestode
Components of the mitogen-activated protein kinase cascade are activated in hepatic cells by Echinococcus multilocularis metacestode
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肝细胞中丝裂原激活蛋白激酶级联的成分被多房棘球绦虫激活
DOI：
10.3748/wjg.15.2116
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发表时间：
2009-05-07
影响因子：
4.3
通讯作者：
Richert, Lysiane
中科院分区：
文献类型：
--
作者：
Lin, Ren-Yong;Wang, Jun-Hua;Richert, Lysiane
目的：为探讨泡球蚴对丝裂原活化蛋白激酶（MAPK）信号通路的激活及对肝细胞增殖的影响，采用免疫组织化学方法检测泡球蚴病（AE）患者肝组织中MAPK磷酸化及增殖细胞核抗原（PCNA）表达的变化。原代培养的大鼠肝细胞在接触（1）多房棘球蚴囊泡液（EmF）和（2）多房棘球蚴条件培养液（EmCM）后，可检测到MAPK、MEK 1/2（MAPK/ERK）和核糖体S6激酶（RSK）磷酸化。在AE患者的肝脏中，ERK 1/2和p38 MAPK被激活，PCNA表达增加，尤其是在后囊蚴附近。暴露于EmF后，p38，c-Jun N-末端激酶（JNK）和ERK 1/2也在体外肝细胞中被激活，以及MEK 1/2和RSK，在没有任何毒性作用的情况下。结论：以往的研究表明，宿主对E.多房群岛我们的数据表明，相反的，即有效地作用于宿主肝细胞中的MAN信号传导途径的帕拉衍生信号实际上正在起作用。(C)2009 WJG出版社和百世登。All rights reserved.
AIM: To explore the effect of Echinococcus multilocularis on the activation of mitogen-activated protein kinase (MAPK) signaling pathways and on liver cell proliferation,METHODS: Changes in the phosphorylation of MAPKs and proliferating cell nuclear antigen (PCNA) expression were measured in the liver of patients with alveolar echinococcosis (AE). MAPKs, MEK1/2 [MAPK/extracellular signal-regulated protein kinase (ERK) kinase] and ribosomal S6 kinase (RSK) phosphorylation were detected in primary cultures of rat hepatocytes in contact in vitro with (1) E multilocularis vesicle fluid (EmF), (2) E multilocularis-conditioned medium (EmCM).RESULTS: In the liver of AE patients, ERK 1/2 and p38 MAPK were activated and PCNA expression was increased, especially in the vicinity of the metacestode. Upon exposure to EmF, p38, c-Jun N-terminal kinase (JNK) and ERK1/2 were also activated in hepatocytes in vitro, as well as MEK1/2 and RSK, in the absence of any toxic effect. Upon exposure to EmCM, only JNK was up-regulated.CONCLUSION: Previous studies have demonstrated an influence of the host on the MAPK cascade in E. multiloculal-is. Our data suggest that the reverse, i.e. para site-derived signals efficiently acting on MAN signaling pathways in host liver cells, is actually operating. (C) 2009 The WJG Press and Baishideng. All rights reserved.