X-ray radiation damage to biological samples: recent progress
X-ray radiation damage to biological samples: recent progress
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DOI：
10.1107/s1600577519009408
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发表时间：
2019-07-01
影响因子：
2.5
通讯作者：
Weik, Martin
中科院分区：
文献类型：
--
作者：
Garman, Elspeth F.;Weik, Martin
在过去的几年中，随着用于大分子晶体学（MX）的光束线的持续发展，提供了越来越高的X射线通量密度，意识到辐射损伤对所产生的结构的影响变得更加重要。本期的九篇论文涵盖了与这种损伤表现的物理和化学相关的一系列方面，如在晶体，溶液和组织样品上的MX和小角X射线散射（SAXS）中所观察到的那样。这些报告包括测量红宝石微晶中X射线照射引起的加热，使用高量子效率的CdTe探测器检查作为入射X射线能量高达30 keV的函数的金属酶的低剂量实验损伤率，以及使用这些探测器预测衍射效率的理论分析，小角X射线散射检查了低剂量辐射照射对与人类健康有关的蛋白质复合物解离的影响，进行了描述辐射化学途径的理论计算，目的是解释在蛋白质中广泛观察到的特定结构损伤，辐射诱导的损伤效应的DNA晶体的调查，一个金属酶的情况下，结构运动被认为是机制相关的可能实际上是辐射损伤诱导的变化，最后一个审查描述什么X射线辐射诱导的半胱氨酸修饰可以教我们有关蛋白质动力学和催化。这些论文，沿着自2017年上一期同步辐射辐射损伤杂志特刊以来发表的其他一些相关文献，简要总结如下。
With the continuing development of beamlines for macromolecular crystallography (MX) over the last few years providing ever higher X-ray flux densities, it has become even more important to be aware of the effects of radiation damage on the resulting structures. Nine papers in this issue cover a range of aspects related to the physics and chemistry of the manifestations of this damage, as observed in both MX and small-angle X-ray scattering (SAXS) on crystals, solutions and tissue samples. The reports include measurements of the heating caused by X-ray irradiation in ruby microcrystals, low-dose experiments examining damage rates as a function of incident X-ray energy up to 30keV on a metallo-enzyme using a CdTe detector of high quantum efficiency as well as a theoretical analysis of the gains predicted in diffraction efficiency using these detectors, a SAXS examination of low-dose radiation exposure effects on the dissociation of a protein complex related to human health, theoretical calculations describing radiation chemistry pathways which aim to explain the specific structural damage widely observed in proteins, investigation of radiation-induced damage effects in a DNA crystal, a case study on a metallo-enzyme where structural movements thought to be mechanism related might actually be radiation-damage-induced changes, and finally a review describing what X-ray radiation-induced cysteine modifications can teach us about protein dynamics and catalysis. These papers, along with some other relevant literature published since the last Journal of Synchrotron Radiation Radiation Damage special issue in 2017, are briefly summarized below.