Altered expression of ADAMS (A Disintegrin And Metalloproteinase) in fibrillating human atria
Altered expression of ADAMS (A Disintegrin And Metalloproteinase) in fibrillating human atria
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DOI：
10.1161/hc0602.103639
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发表时间：
2002-02-12
期刊：
影响因子：
37.8
通讯作者：
Goette, A
中科院分区：
文献类型：
--
作者：
Arndt, M;Lendeckel, U;Goette, A
背景-ADAMS（A Disintegrin And Metalloproteinase）是一种在心肌组织中表达的胞外蛋白酶。虽然已知它们调节细胞-细胞和细胞-基质相互作用，但它们在各种心脏疾病中的病理生理作用尚不清楚。本研究的目的是确定心房颤动（AF）过程中心房的结构重塑是否与ADAM表达的改变有关。方法和结果：对30例接受心脏直视手术的患者的心房组织样本进行了检查。15例患者有持续性房颤（≥ 6个月），其余15例患者无房颤病史。从mRNA和蛋白水平定量分析ADAM 9、ADAM 10和ADAM 15的表达。免疫组化定位ADAM表达。ADAM表达与整合素β 1和β 3的量相关。房颤期间，ADAM 10蛋白的量增加了一倍多（82 +/- 15对36 +/- 8 U; P
Background-ADAMS (A Disintegrin And Metalloproteinase) are ectoproteases that have recently been reported to be expressed in cardiac tissue. Although they are known to regulate cell-cell and cell-matrix interactions, their pathophysiological role in various cardiac diseases is unclear. The purpose of the present study was to determine whether structural remodeling of the atria during atrial fibrillation (AF) is associated with altered ADAM expression.Methods and Results-Atrial tissue samples of 30 patients undergoing open-heart surgery were examined. Fifteen patients had persistent AF (greater than or equal to6 months), and the remaining 15 patients had no history of AF. ADAM9, ADAM 10, and ADAM 15 expression was analyzed quantitatively at the mRNA and protein levels. ADAM expression was localized by immunohistochemistry. ADAM expression was correlated with amounts of integrins beta(1) and beta(3). The amount of ADAM10 protein more than doubled during AF (82 +/- 15 versus 36 +/- 8 U; P