Inositol based non-viral vectors for transgene expression in human cervical carcinoma and hepatoma cell lines.
Inositol based non-viral vectors for transgene expression in human cervical carcinoma and hepatoma cell lines.
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DOI：
10.1016/j.biomaterials.2013.11.034
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发表时间：
2014-02
期刊：
影响因子：
14
通讯作者：
Lei Zhang;Yiwen Fan;Yunkun Wu
中科院分区：
文献类型：
--
作者：
Lei Zhang;Yiwen Fan;Yunkun Wu
肌醇（Myo-Inositol， INO）是一种具有多种功能的生物分子。在本研究中，以肌醇和低分子量聚乙烯亚胺为原料合成了用于基因传递的超支化共聚物。证明了INO-PEIs装载质粒DNA的能力及其生物相容性。广泛用于药物传递系统的肿瘤靶配体叶酸（FA）随后被偶联到ino - pei上，从而形成INO-PEI-FA共聚物。然后评估聚合物在哺乳动物细胞系中介导转基因表达的活性。如所示，INO-PEIs能够介导高效的转基因表达，这在癌细胞系HeLa中尤为明显。INO-PEI-FA进一步提高了HepG2的效率。讨论了INO-PEI-FA聚合物在非癌性NIH 3T3和癌性HeLa细胞系中的分布。
Myo-Inositol (INO) is a biomolecule with crucial functions in many aspects. In this study, hyperbranched copolymers for gene delivery were synthesized based on inositol and low molecular weight polyethylenimine. The capacity of INO-PEIs to load plasmid DNA and their biocompatibility was demonstrated. A tumor target ligand, folic acid (FA), which was widely used for drug delivery systems, was subsequently conjugated to INO-PEIs and resulted in INO-PEI-FA copolymers. The polymers were then evaluated on their activity to mediate transgene expression in mammalian cell lines. As indicated, INO-PEIs were able to mediate efficient transgene expression, which was particularly noticeable in carcinoma cell line HeLa. INO-PEI-FA further improved the efficiency in HepG2. Distribution of INO-PEI-FA polymers in non-carcinoma NIH 3T3 and carcinoma HeLa cell lines was discussed.