Highly specific detection of IL-8 protein using combination tapered fiber-optic biosensor dip-probe.
Highly specific detection of IL-8 protein using combination tapered fiber-optic biosensor dip-probe.
复制标题
使用组合锥形光纤生物传感器浸入式探针对 IL-8 蛋白进行高度特异性检测。
DOI：
10.1117/12.843284
复制
发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Kapoor,Rakesh
中科院分区：
文献类型：
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作者：
Wang,Chun-Wei;Kapoor,Rakesh
我们正在报告检测IL-8的混合蛋白质溶液中，使用组合锥形光纤生物传感器（CTFOB）浸渍探针。检测方法采用夹心免疫法。通过使用两种类型的阴性对照探针确定传感器的特异性。结果表明，利用CTFOB双探针可成功地检测混合蛋白中的IL-8，且具有较高的特异性。IL-8的最低检测浓度为150 pM。
We are reporting detection of IL-8 in a mixed protein solution, using combination tapered fiber-optic biosensor (CTFOB) dip-probe. Sandwich immunoassay was used as the detection technique. The specificity of the sensor was established by using two types of negative control probes. It is demonstrated that with the help of these CTFOB dipprobe we could successfully detect IL-8 with high specificity in protein mixture. The lowest detected concentration of IL-8 was 150 pM.