Potassium recycling in the renal medulla: effects of acute potassium chloride administration to rats fed a potassium-free diet.
Potassium recycling in the renal medulla: effects of acute potassium chloride administration to rats fed a potassium-free diet.
复制标题
肾髓质中的钾循环：急性氯化钾给药对无钾饮食喂养的大鼠的影响。
DOI：
10.1038/ki.1981.145
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发表时间：
1981
影响因子：
19.6
通讯作者：
Jamison,RL
中科院分区：
文献类型：
--
作者：
Arrascue,JF;Dobyan,DC;Jamison,RL
方法14只雄性和雌性Munich-Wistar大鼠，体重70 ~ 110 g。给动物喂食商业缺钾饮食（ICN Pharmaceuticals，Inc.，Cleveland，俄亥俄州;目录号904665）。在实验当天，用mactin（100 mg/kg体重）（Henly and Company，纽约，纽约）麻醉动物，并如前所述准备左肾乳头的显微穿刺[31.通过膀胱导管收集右未暴露肾脏的尿液。动物接受含有足够量菊粉的0.9%盐水（0.93 ml/min）的连续输注，以将血浆水平维持在60至100 mg/dl。在对照收集尿液后，用吸头直径为5 - 7微米的移液管收集靠近延髓降支末端2 - 6 mm的小管液[3]。然后改变输注，根据输注情况将动物分为两组：I组。8只大鼠接受菊粉和盐水的输注，其中还含有氯化钾，以3.75 mEq/min/kg体重的速率输注。此后，在接下来的120至160 mm内采集末端降支液和尿液样本。我们的目标是尝试以20 mm的间隔采集成对的降支液和尿液样本。由于技术原因，不可能精确地每隔20 mm定位和穿刺一个环并不间断地收集足够的小管液用于分析。但是，在输注氯化钾后，以20 5 mm或其倍数的间隔获得了1 oop Henle样本的四分之三。
Methods Fourteen young male and female Munich-Wistar rats each weighing between 70 and 110 g, were used in this study. The animals were fed a commercial potassium-deficient diet (ICN Pharmaceuticals, Inc., Cleveland, Ohio; catalog no. 904665) for3 days prior to the day of the study. On the day of the experiment, the animals were anesthetized with mactin (100 mg/kg of body wt)(Henly and Company, New York, New York) and prepared for micropuncture of the left renal papilla as previously described [31. Urine from the right unexposed kid-ney was collected through a bladder catheter. The animals received a continuous infusion of 0.9% saline (0.93 ml/min) containing inulin in sufficient quantity to maintain plasma levels at 60 to 100 mg/dl. After a control collection of urine, tubule fluid was collected for 2 to 6 mm near the end of the juxtamedullary descending limb with pipettes having a tip diameter of 5 to 7 microns [3]. Then the infusion was changed, according to which the animals were divided into two groups: Group I. Eight rats received the infusion of inulin and saline which, in addition, contained potassium chloride, infused at a rate of 3.75 mEq min. kg body wr'. Thereafter, samples of end-descending limb fluid and urine were obtained for the next 120 to 160 mm. Our goal was to try to collect paired descending limb fluid and urinary samples at 20 mm intervals. For technical reasons, it was impossible to locate and puncture a loop and collect uninterruptedly sufficient tubule fluid for analyses pre-cisely each 20 mm. But, three-fourths of the 1oop of Henle samples were obtained at intervals of 20 5 (range) mm, or multiples thereof, after the infusion of potassium chloride was